Journal ArticleDOI
Molecular weights of RNA segments of influenza A and B viruses
Ulrich Desselberger,Peter Palese +1 more
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TLDR
The RNAs of different influenza viruses were analyzed together with marker RNAs on polyacrylamide-urea gels after denaturation with glyoxal to suggest that the size of the HA and NA genes may vary among different human and animal influenza A viruses.About:
This article is published in Virology.The article was published on 1978-07-15. It has received 55 citations till now. The article focuses on the topics: Neuraminidase & RNA.read more
Citations
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Journal ArticleDOI
Evidence for a ninth influenza viral polypeptide
TL;DR: The available evidence suggests that the synthesis of polypeptide 4 requires “early” protein synthesis, which is distinct from the eight defined influenza virus gene products.
Journal ArticleDOI
Cloning of influenza cDNA into M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein
Greg Winter,Stanley Fields +1 more
TL;DR: A strategy has been developed for sequencing the single-stranded RNA genes of influenza virus and the nucleotide sequence of segment 7 was completed and provides the primary structure of the matrix protein.
Journal ArticleDOI
Nucleotide sequence heterogeneity of the RNA from a natural population of foot-and-mouth-disease virus.
TL;DR: Oligonucleotide mapping indicates that the variant oligonucleotides are scattered throughout the FMDV genome, suggesting that extensive genetic variability at many RNA sites is the basis for the antigenic diversity of F MDV.
Journal ArticleDOI
The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities
TL;DR: A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV-1 cells, which suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity.
Book ChapterDOI
The Influenza Virus RNA Segments and Their Encoded Proteins
TL;DR: A large body of evidence concerning changes in the surface glycoproteins, the hemagglutinin and neuraminidase, has been obtained from studies on the primary sequences, interactions with antibodies and the correlation of these data with the three-dimensional X-ray structure of these proteins.
References
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Journal ArticleDOI
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
TL;DR: RNA molecular weight measurements were carried out by gel electrophoresis under four different denaturing conditions including 99% formamide, 10 mM methyl mercury, 2.2 M formaldehyde, and 6 M urea at pH 3.8 to demonstrate reliable molecular weight determinations of denatured RNAs, especially those obtained by extrapolation.
Journal ArticleDOI
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
James M. Bailey,Norman Davidson +1 more
TL;DR: It appears that complete denaturation of any base-paired secondary structural feature of a nucleic aicd can be achieved at practical concentrations of CH3HgOH.
Journal ArticleDOI
Differences in RNA patterns of influenza A viruses.
Peter Palese,Jerome L. Schulman +1 more
TL;DR: Analysis of the segmented RNAs of influenza A viruses by electrophoresis on polyacrylamide urea slab gels has provided a method for sharper resolution of the number and migration rates of different segments, and may be possible to define which RNA segment codes for each viral protein.
Journal ArticleDOI
Polyacrylamide gel electrophoresis of influenza virus RNA.
Marcel W. Pons,George K. Hirst +1 more
Journal ArticleDOI
Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein.
TL;DR: This report has identified the genes coding for the remaining virus-specific moving RNA segment of influenza A/PR/8/34 (HON1) virus and segment 6 of Hong Kong virus coded for the respective nucleoproteins, and that segment 7 of both viruses codes for the membtane protein and RNA segment 8 codes forThe nonstructural protein, completing the mapping of the influenza A virus genome.