Showing papers in "Virology in 1987"

TL;DR: The shell of the tubular core shows p24 antigenicity, while p17 is located at the inner leaflet of the lipid membrane, and the virus particle is studded with 70–80 protrusions.

TL;DR: Studies with a third mutant virus, pm2022, suggest that very low levels of 495R protein activity are sufficient for a productive infection and significant transforming activity, and provide evidence that the observed transformation defect is not due to the death of transformed cells.

TL;DR: The complete nucleotide sequence of the Japanese encephalitis virus (JEV) genome RNA was determined and it was found that the C-terminal region of the ORF may encode a RNA-dependent RNA polymerase which has significant sequence homology with those of other RNA viruses.

TL;DR: Comparing 150 bases of genomic sequence information from 62 isolates obtained from poliomyelitis patients in five continents allowed us to identify numerous geographic foci of endemic circulation of wild type 1 polioviruses, reveal previously unsuspected links between cases in distant communities, and recognize the recombinant (vaccine-wild; wild-wild) origins of some epidemic poliovIRuses.

TL;DR: Using high resolution electron microscopic autoradiography and immunocytochemistry with monoclonal antibodies against poliovirus proteins of the P2 genomic region, the location of these proteins in respect to the virus-induced vesicle formation and the viral RNA synthesis was followed during the viral replication cycle.

TL;DR: The 30K protein of tobacco mosaic virus was localized to the plasmodesmata of infected tobacco leaves by immunogold cytochemistry, and no specific label was found in the nuclei or at any other site in the cells.

TL;DR: Deletion mapping studies suggest that VP4 is involved in the processing of the precursor polyprotein, and the conformational epitope recognized by the virus neutralizing monoclonal antibody is present within VP2.

TL;DR: Lower doses of P2 still provided a significant level of protection even though no neutralizing antibodies could be detected, and sheep were fully protected against challenge with a virulent strain of the same BTV serotype.

TL;DR: The majority of the latent HSV-1 DNA exists in an extrachromosomal state in the mouse model by CsCl buoyant density gradient centrifugation.

TL;DR: The importance of the dibasic residues for efficient proteolytic activation of the fusion protein and for the pantropic property of NDV is proposed.

TL;DR: On integration, the E2 open reading frame (ORF) was invariably lost but the E6/E7 ORFs and the long control region of the HPV-16 genome were retained in all seven integrations analyzed and may play a role in cellular transformation and/or maintenance of the transformed phenotype.

TL;DR: The M genome segment of Hantaan virus was molecularly cloned and the nucleotide sequence of cDNA was determined, revealing a gene order with respect to message sense RNA of 5'-G1-G2-3'.

TL;DR: Effective purification methods have been developed for virus particles, infectious subviral particles (ISVP), and virus cores of bluetongue virus (BTV) serotypes 1 and 4 and the purified virus particles of both serotypes were consistently found to contain small amounts of BTV protein NS2, previously regarded as exclusively nonstructural.

TL;DR: The predicted amino acid sequences of the polyproteins of two potyviruses, TVMV and TEV, were compared to each other, to proteins of other viruses, and to the National Biomedical Research Foundation protein sequence bank as discussed by the authors.

TL;DR: Molecular changes in the HA of an A(H1N1) virus which accompany egg adaptation suggest that egg-adapted variants are selected on the basis of altered receptor site specificity with concomitant effects on the antigenic configuration of the HA molecule.

TL;DR: One of the viruses is a candidate vector for insertion of foreign genes into the chicken germ line and transmitted from G-1 to the G-2 progeny in a Mendelian fashion thus proving that retroviral genes have been inserted into theChicken germ line.

TL;DR: The entire nucleotide sequence of the coxsackievirus B3 strain Nancy (CB3) genome has been determined from cDNA and a detailed comparison was carried out between the proteins encoded by the CB3 and poliovirus type 1 strain Mahoney genomes.

TL;DR: Analysis of cDNA clones prepared from the RNA of cells replicating EBV revealed that both RNAs contain the BHRF1 open reading frame, and the predicted amino acid sequence of the protein has characteristics which suggest that it is a membrane protein.

TL;DR: Fusion of BMV cDNA directly to the initiation site of the canonical phi10 promoter of bacteriophage T7 allows efficient in vitro synthesis of infectious BMV transcripts by T7 RNA polymerase, providing substantial improvement in the ease and reproducibility with which BMv cDNA can be expressed.

TL;DR: Amino acid sequence comparison of the A59 and 1HM E2 proteins showed extensive homology and revealed a stretch of 89 amino acids in the 90B region ofThe A59 E2 protein that is absent in JHM.

TL;DR: HIV-infected U937 cells retain the ability to differentiate, though less efficiently, as shown by the appearance of monocyte/macrophage surface markers, following TPA treatment.

TL;DR: Putative nonstructural proteins NS2a, NS2b, NS4a, and NS4b are extremely hydrophobic, suggesting that these proteins are most likely associated with cellular membranes.

TL;DR: A complete set of recombinant vaccinia viruses that express each of the influenza virus polypeptides has been constructed and direct immunofluorescence studies have shown that HA, NA, and MS2 proteins migrate to the cell surface; PB2, PB1, PA, NP, and NS1 proteins migrateto the cell nucleus; and M1 and NS2 are distributed throughout the cell, although NS2 accumulates preferentially in nuclei.

TL;DR: The complete nucleotide sequence of the genome of the coxsackievirus B1, a human enterovirus that belongs to the Picornaviridae, was determined by using molecular cloning and rapid sequence analysis techniques to predict precise map locations of the viral structural and nonstructural proteins on the genome.

TL;DR: A higher degree of amino acid sequence homology was found between the M proteins of BCV and MHV than between the N proteins, and notable differences were found at the amino terminus, the most probable site of O-glycosylation.

TL;DR: Investigating the influence of the host cell on the selection of antigenic variants of influenza A H3N2 viruses and the relevance of host cell selection to the induction of immunity by these viruses found ferrets infected with either live egg-grown or MDCK-grown virus were protected equally well from challenge with virus grown in either host cell type.

TL;DR: The major glycoproteins gI, gIII, and gIV of bovine herpesvirus-1 were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of BHV-1-infected cells.

TL;DR: Results indicate that the delay in disease development includes prevention of virus accumulation in the inoculated leaves, and the protection observed for the transgenic plants is referred to as "genetically engineered cross-protection".

TL;DR: Observations demonstrate that coinfecting bacteria can play an essential role in the development of influenza pneumonia by providing a protease suitable for cleavage activation of the hemagglutinin.

TL;DR: The observation of viral-cell transcripts and chromosomal deletions associated with HPV integration may indicate that such events are part of a multistep mechanism leading to the development of cervical cancer.