Influenza virus genome consists of eight distinct RNA species
TLDR
The genomic RNA of the avian influenza A virus, fowl plague, was fractionated into eight species by electrophoresis in polyacrylamide-agarose gels containing 6 M urea and it was demonstrated that each species has a distinct nucleotide sequence.Abstract:
The genomic RNA of the avian influenza A virus, fowl plague, was fractionated into eight species by electrophoresis in polyacrylamide-agarose gels containing 6 M urea. The separated 32P-labeled RNA species were characterized by digestion with RNase T1 and fractionation of the resulting oligonucleotides by two-dimensional gel electrophoresis; this demonstrated that each species has a distinct nucleotide sequence. A tentative correlation of each genome RNA species with the virus protein that it encodes was made.read more
Citations
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On the origin of the human influenza virus subtypes H2N2 and H3N2
TL;DR: The RNA of the human influenza virus Singapore (H2N2) strain has been labeled in vivo by phosphorus-32 and separated by polyacrylamide gel electrophoresis into eight segments, which were correlated to the corresponding gene functions and/or proteins.
Journal ArticleDOI
Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement.
TL;DR: The aim of this review is to present the current mechanistic understanding for how IAVs facilitate cell entry, replication, virion assembly, and intercellular movement, in an effort to highlight some of the unanswered questions regarding the coordination of the IAV infection process.
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The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity.
TL;DR: The 3'- and 5'-terminal nucleotides of the genome segments of an influenza A, B, and C virus were identified by directly sequencing viral RNA using two different sequencing techniques and showed partial and inverted complementarity.
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Genome packaging in influenza A virus
TL;DR: It is concluded that the vast majority of virions contain no more than eight segments and that a specific mechanism does indeed function to select one copy of each vRNA.
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Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.
Robert A. Lamb,Ching-Juh Lai +1 more
TL;DR: The complete sequence of cloned full-length DNA (NS DNA) derived from influenza virus gene 8, which codes for two unique polypeptides, NS1 and NS2, and the sequence of the NS2 mRNA is obtained, indicating that NS1and NS2 overlap by 70 amino acids that are translated from different reading frames.
References
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Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels
A C Peacock,C W Dingman +1 more
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Molecular weights of ribosomal RNA in relation to evolution.
TL;DR: The results thus confirm the sharp distinction between the RNA of prokaryotic and eukaryotic organisms and show that there has been a strong conservation of molecular size during evolution, and suggest that the increased size of the animal ribosome was essential for animal evolution.
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Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA
R. De Wachter,Walter Fiers +1 more
TL;DR: The method has been applied to the complete separation of RNA fractions obtained after a preliminary gel electrophoresis of partial enzymic digests of 32P-labeled bacteriophage RNA.