Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis
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This article presents a review of recent laboratory-developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests and focuses on two commercial syndromic multiplex tests: Luminex xTAG Gastrointestinal Pathogen Panel and BioFire FilmArray gastrointestinal test.About:
This article is published in Clinics in Laboratory Medicine.The article was published on 2015-06-01 and is currently open access. It has received 109 citations till now. The article focuses on the topics: Multiplex polymerase chain reaction & Multiplex.read more
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Clinical Practice Guidelines for Clostridium difficile Infection in Adults and Children: 2017 Update by the Infectious Diseases Society of America (IDSA) and Society for Healthcare Epidemiology of America (SHEA)
L. Clifford McDonald,Dale N. Gerding,Stuart Johnson,Stuart Johnson,Johan S. Bakken,Karen C. Carroll,Susan E. Coffin,Erik R. Dubberke,Kevin W. Garey,Carolyn V. Gould,Ciaran P. Kelly,Vivian G. Loo,Julia S. Sammons,Thomas J. Sandora,Mark H. Wilcox +14 more
TL;DR: This guideline updates recommendations regarding epidemiology, diagnosis, treatment, infection prevention, and environmental management on Clostridium difficile infection in adults and includes recommendations for children.
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Depletion of Human DNA in Spiked Clinical Specimens for Improvement of Sensitivity of Pathogen Detection by Next-Generation Sequencing.
Mohammad Rubayet Hasan,Arun Rawat,Patrick Tang,Puthen V. Jithesh,Eva Thomas,Rusung Tan,Peter Tilley +6 more
TL;DR: A specimen-processing protocol to remove human DNA and enrich specimens for bacterial and viral DNA for shotgun metagenomic sequencing and significantly decreased NGS reads mapped to the human genome and improved the sensitivity of pathogen detection.
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Multiplex Molecular Panels for Diagnosis of Gastrointestinal Infection: Performance, Result Interpretation, and Cost-Effectiveness
TL;DR: This review will focus on the multiplex molecular panels that have received clearance from the FDA for the diagnosis of diarrheal disease and will highlight issues related to test performance, result interpretation, and cost-effectiveness of these new molecular diagnostic tools.
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A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics
TL;DR: The utility of chromogenic media in clinical microbiology is explored, with particular attention given to media that have been commercialized in the last decade, and the role of laboratory automation and complementary technologies such as matrix-assisted laser desorption ionization–time of flight mass spectrometry are assessed.
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New technologies for detection of enteric parasites
TL;DR: New commercially available enteric panel assays, which detect parasites (as well as bacteria and/or viruses) using multiplex PCR, offer enhanced sensitivity and specificity as well as the ability to detect mixed infections, and will play an important role in epidemiological surveillance and outbreak investigations.
References
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