Journal ArticleDOI
Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1.
J. Kruse,R. Mailhammer,H Wernecke,Andreas Faissner,Iris E. C. Sommer,Christo Goridis,Melitta Schachner +6 more
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It is shown here that a carbohydrate moiety recognized by L2 and HNK-1 monoclonal antibodies, is present in mouse N-CAM and LI, which points to a previously undetected molecular diversity which may have functional implications for modulating cell adhesion during development.Abstract:
Cell surface molecules have been implicated in cell interactions which underlie formation of the nervous system. The analysis of the functional properties of such molecules has profited from the combined use of antibodies and cell culture systems. It has been suggested that the interplay between these molecules modulates cell-to-cell interaction at critical developmental stages. In the mouse, N-CAM and L1 antigen have been shown to mediate Ca2+-independent adhesion among neural cells. N-CAM plays a role in fasciculation of neurites and formation of neuromuscular junction. L1 is apparently not involved in synaptogenesis, but in migration of granule cell neurones in the developing mouse cerebellar cortex. The two antigens are distinct molecular and functional entities which act synergistically in aggregation of neuroblastoma and early postnatal cerebellar cells. In view of a certain similarity in function between the two groups of molecules, it was not surprising to find that structural similarities are detectable by the monoclonal antibody L2. We show here that a carbohydrate moiety recognized by L2 and HNK-1 monoclonal antibodies, is present in mouse N-CAM and L1. The L2 epitope appears on all major neural cell types but not all N-CAM molecules express it. This heterogeneity points to a previously undetected molecular diversity which may have functional implications for modulating cell adhesion during development.read more
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Biology of natural killer cells.
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The J1 glycoprotein--a novel nervous system cell adhesion molecule of the L2/HNK-1 family.
TL;DR: Polyclonal antibodies are prepared to a prominent member of the L2/HNK-1 family and it is reported that these antibodies, designated J1 antibodies, react with astrocytes and oligodendroCytes and interfere with neurone–astrocyte adhesion, but not with neur one–neurone or astroCyte–astrospecific adhesion.
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Glioma invasion in the central nervous system.
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Journal ArticleDOI
Differential inhibition of neurone-neurone, neurone-astrocyte and astrocyte-astrocyte adhesion by L1, L2 and N-CAM antibodies.
TL;DR: It is reported that LI antigen promotes neurone–neurone adhesion and the L2 carbohydrate epitope shared between the two adhesion molecules seems to be involved in neurones and glia, and acts in a more than additive manner in N-CAM-mediated neurone-neur one adhesion.
References
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Journal Article
A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
T. Abo,Charles M. Balch +1 more
TL;DR: The monoclonal antibody HNK-1, produced against a membrane antigen from the cultured T cell line, HSB-2, defines the first differentiation antigen shown to be selectively expressed on human NK and K cells.
Journal ArticleDOI
Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: An immunocytological study in the central nervous system
TL;DR: In this article, four monoclonal antibodies are characterized from a fusion of mouse myeloma P3-NS1/1-Ag4-1 with spleen cells from BALB/c mice immunized with white matter from bovine corpus callosum.
Journal ArticleDOI
Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion.
TL;DR: During cerebellar development L1 antigen is detectable on tetanus toxin‐positive cells as early as embryonic day 13 after 3 days in culture and in the adult cerebellum, where it is predominantly localized in the molecular layer and around Purkinje cells.
Journal ArticleDOI
Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system.
TL;DR: The results show that vimentin and GFA protein coexist in one cell type not only in primary cultures in vitro but also in the intact tissue in situ.
Journal ArticleDOI
endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.
John H. Elder,Stephen Alexander +1 more
TL;DR: The data indicate that cleavage occurs via hydrolysis of the glycosidic bond of the N,N'-diacetylchitobiose core structure adjacent to asparagine, similar to that due to endo H and endo D.
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