Journal ArticleDOI
New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry.
Rustam Ziganshin,Georgii Arapidi,I. V. Azarkin,Elena Zaryadieva,Dmitry G. Alexeev,Vadim M. Govorun,Vadim T. Ivanov +6 more
Reads0
Chats0
TLDR
Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI-TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases theNumber of identified blood serum peptides by LC-MS/MS using an Agilent 6520 Accurate-Mass Q-TOf.About:
This article is published in Journal of Proteomics.The article was published on 2011-05-01. It has received 22 citations till now. The article focuses on the topics: Blood serum & Protein mass spectrometry.read more
Citations
More filters
Analysis of albumin-associated peptides and proteins from ovarian cancer patients
Mark S. Lowenthal,Arpita I. Mehta,Kristina Frogale,Russell W. Bandle,Robyn P. Araujo,Brian L. Hood,Timothy D. Veenstra,Thomas P. Conrads,Paul K. Goldsmith,David A. Fishman,Emanuel F. Petricoin,Lance A. Liotta +11 more
TL;DR: In this paper, an experimental method to isolate albumin in its native state and then identify [mass spectrometry (MS) sequencing] the corresponding bound low-molecular-weight molecules was developed.
Journal ArticleDOI
Semi-quantitative analysis of changes in the plasma peptidome of Manduca sexta larvae and their correlation with the transcriptome variations upon immune challenge.
TL;DR: Fat body contributed a significant portion of the plasma polypeptides involved in various aspects of innate immunity after the bacterial injection, as judged by significant changes in normalized spectral counts between the control and induced plasma.
Journal ArticleDOI
An update for human blood plasma pretreatment for optimized recovery of low-molecular-mass peptides prior to CE–MS and SPE–CE–MS.
TL;DR: Recoveries and repeatability were only optimum after passivating the 10 K Mr cut-off cellulose acetate filters with PEG to avoid peptide adsorption on the inner walls of the plastic sample reservoir.
Journal ArticleDOI
Microchip Emitter for Solid-Phase Extraction−Gradient Elution−Mass Spectrometry
TL;DR: Fortified human blood serum, protein digest and fractions collected after protein digest OFFGEL separation were analyzed by SPE-GEMS allowing the detection of low abundance peptides usually not observed by direct mass spectrometry analysis.
Journal ArticleDOI
Proteomic Analysis of Polypeptides Captured from Blood during Extracorporeal Albumin Dialysis in Patients with Cholestasis and Resistant Pruritus
TL;DR: The peptides and proteins absorbed into the MARS strong anion exchange (SAX) cartridges as a result of the treatment of patients with cholestasis and resistant pruritus and confirmed to be removed by the dialysis procedure using targeted selected-reaction monitoring MS/MS.
References
More filters
Journal ArticleDOI
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
TL;DR: Silver staining allows a substantial shortening of sample preparation time and may, therefore, be preferable over Coomassie staining, and this work removes a major obstacle to the low-level sequence analysis of proteins separated on polyacrylamide gels.
Journal ArticleDOI
The Human Plasma Proteome History, Character, and Diagnostic Prospects
TL;DR: This work speculates on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggests approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.
Journal ArticleDOI
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
TL;DR: StageTips are ordinary pipette tips containing very small disks made of beads with reversed phase, cation-exchange or anion-ex exchange surfaces embedded in a Teflon mesh that allow flexible combination of disks with different surfaces to obtain multi-functional tips.
Journal ArticleDOI
Proteomics and Bioinformatics Approaches for Identification of Serum Biomarkers to Detect Breast Cancer
TL;DR: Proteomics approaches such as SELDI mass spectrometry, in conjunction with bioinformatics tools, could greatly facilitate the discovery of new and better biomarkers and show great potential for the early detection of breast cancer.
Journal ArticleDOI
Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry.
TL;DR: The overall history and recent advances in surface enhanced laser desorption/ionization‐time of flight‐mass spectrometry (SELDI‐TOF‐MS) technology is reviewed and its application to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma.