Journal ArticleDOI
Nitrile Metabolizing Enzymes in Biocatalysis and Biotransformation.
TLDR
A number of opportunities and challenges of nitrile metabolizing enzymes in bioprocess development for the production of bulk and fine chemicals are discussed.Abstract:
Nitrile metabolizing enzymes, i.e., aldoxime dehydratase, hydroxynitrile lyase, nitrilase, nitrile hydratase, and amidase, are the key catalysts in carbon nitrogen triple bond anabolism and catabolism. Over the past several years, these enzymes have drawn considerable attention as prominent biocatalysts in academia and industries because of their wide applications. Research on various aspects of these biocatalysts, i.e., sources, screening, function, purification, molecular cloning, structure, and mechanisms, has been conducted, and bioprocesses at various scales have been designed for the synthesis of myriads of useful compounds. This review is focused on the potential of nitrile metabolizing enzymes in the production of commercially important fine chemicals such as nitriles, carboxylic acids, and amides. A number of opportunities and challenges of nitrile metabolizing enzymes in bioprocess development for the production of bulk and fine chemicals are discussed.read more
Citations
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Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis (vol 72, pg 77, 2006)
TL;DR: An inducer feeding approach is suggested to achieve enhanced enzyme production and therefore higher degree of conversion of mandelonitrile, using immobilized cells of Pseudomonas putida MTCC 5110 harbouring a stereoselective nitrilase.
Journal ArticleDOI
Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications
Francisco Thálysson Tavares Cavalcante,Italo Rafael de Aguiar Falcão,José Erick da Silva Souza,Thales Guimaraes Rocha,Isamayra Germano de Sousa,Antônio Luthierre Gama Cavalcante,André L. B. de Oliveira,Maria C. M. de Sousa,José C. S. dos Santos +8 more
TL;DR: To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials.
Journal ArticleDOI
A review on remediation of cyanide containing industrial wastes using biological systems with special reference to enzymatic degradation
TL;DR: The origin and environmental distribution of cyanide waste along with different bioremediation pathways and enzymes involved therein are discussed, which will help to recoup the environment from cyanide effluent.
Journal ArticleDOI
Biological Properties of Vitamins of the B-Complex, Part 1: Vitamins B1, B2, B3, and B5
Marcel Hrubša,Tomáš Siatka,Iveta Nejmanová,Marie Vopršalová,Lenka Kujovská Krčmová,Kateřina Matoušová,Lenka Javorská,Kateřina Macáková,Laura Mercolini,Fernando Remião,Marek Matus,Přemysl Mladěnka,On Behalf Of The Oemonom +12 more
TL;DR: This review summarizes the current knowledge on essential vitamins B1, B2, B3, and B5, which are water soluble and generally very safe, and their physiological function, which is mainly limited to hypovitaminoses or similar conditions.
Journal ArticleDOI
Nitrilase: a promising biocatalyst in industrial applications for green chemistry.
TL;DR: An overview of recent advances of nitrilases in aspects of distribution, enzyme screening, molecular structure and catalytic mechanism, protein engineering, and their potential applications in industry is provided.
References
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Journal ArticleDOI
Nitrile hydratase and its application to industrial production of acrylamide.
TL;DR: This review summarizes the history of NHase, which has been used for the industrial production of acrylamide from acrylonitrile, the first successful example of a biotransformation process for the manufacture of a commodity chemical.
Journal ArticleDOI
Flagella and bacterial pathogenicity
TL;DR: This mini‐review summarizes data from recent studies that elucidated how flagella, as a virulence factor, contribute to bacterial pathogenicity.
Journal ArticleDOI
The nitrilase superfamily: classification, structure and function
Helen C. Pace,Charles Brenner +1 more
TL;DR: The nitrilase superfamily consists of thiol enzymes involved in natural product biosynthesis and post-translational modification in plants, animals, fungi and certain prokaryotes and genetic and biochemical analysis of the family members and their associated domains assists in predicting the localization, specificity and cell biology of hundreds of uncharacterized protein sequences.
Journal ArticleDOI
The nitrilase family of CN hydrolysing enzymes - a comparative study.
TL;DR: This review provides an overview of current understanding of the genetics and biochemistry of this interesting group of enzymes, which show significant similarities at the amino acid and protein structure level but show many differences in catalytic capability.
Journal ArticleDOI
Cyanogenesis in plants and arthropods.
TL;DR: The transfer of a nuptial gift of cyanogenic glucosides during mating of Zygaena has been demonstrated as well as the involvement of hydrogen cyanide in male attraction and nitrogen metabolism.