Nitrogen Fixation and Co-oxidation of Ethylene by a Methane-utilizing Bacterium
J.A.M. de Bont,E. G. Mulder +1 more
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In this paper, a methane-oxidizing bacterium, isolated from soil, was capable of fixing nitrogen, but not when growing on methanol, although 15N2 was fixed.Abstract:
SUMMARY: A methane-oxidizing bacterium, isolated from soil, was capable of fixing nitrogen. Nitrogenase activity could be assayed by acetylene reduction when the bacterium was growing on methanol but not when growing on methane, although 15N2 was fixed. Bacteria growing on methane co-oxidized ethylene but methanol-growing cells did not. The organism was extremely sensitive to oxygen when dependent on N2 as nitrogen source, a consequence of the sensitivity of its nitro-genase towards oxygen.read more
Citations
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Biogeochemical aspects of atmospheric methane
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Use of “Specific” Inhibitors in Biogeochemistry and Microbial Ecology
TL;DR: The term “specific inhibitor” has been applied to these types of compounds when they are used to probe the functions of mixed populations of microorganisms, providing powerful experimental tools for investigating the activity and function of certain types of micro organisms in natural samples.
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Effects of O2 and CH4 on presence and activity of the indigenous methanotrophic community in rice field soil
TL;DR: Type I methanotrophs responded fast and with pronounced shifts in population structure and dominated the activity under all four gas mixtures, although apparently more abundant, always present and showing a largely stable population structure.
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Aquatic acetylene-reduction techniques: solutions to several problems.
TL;DR: A refinement of these older techniques is introduced and problems that it overcomes are also discussed, and a depth profile of nitrogen fixation (C2H4 production) is shown.
References
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Journal ArticleDOI
Enrichment, isolation and some properties of methane-utilizing bacteria.
TL;DR: The organisms were classified into five groups on the basis of morphology, fine structure, and type of resting stage formed (exospores and different types of cysts) and into subgroups on other properties.
Journal ArticleDOI
Applications of the acetylene-ethylene assay for measurement of nitrogen fixation
TL;DR: The biochemical basis of the assay is described along with relevant characteristics including Km, C2H2/N2 conversion factor, and specific N2[C2H 2]-fixing activities obtained with various systems, and methods of measurement of N2 fixation are compared.
Journal ArticleDOI
Effect of Oxygen on Growth of Azotobacter chroococcum in Batch and Continuous Cultures
H. Dalton,J. R. Postgate +1 more
TL;DR: In Initiation of growth of nitrogen-fixing Azotobacter species was prevented by efficient aeration but proceeded normally with gentle aeration; addition of CO2 to the air did not relieve inhibition, suggesting two mechanisms exist in the cell to protect the oxygen-sensitive components of nitrogenase from oxygen.
Journal ArticleDOI
Alternative Carbon Assimilation Pathways in Methane-utilizing Bacteria
A. J. Lawrence,J. R. Quayle +1 more
TL;DR: The examination is based on the distribution of two key enzymes, each of which appears to be specifically involved in one of the assimilation pathways, namely hydroxypyruvate reductase (serine pathway) and hexose phosphate synthetase (ribose phosphate cycle).
Journal ArticleDOI
Nitrogen Fixing Bacteria
TL;DR: According to LIGNIER, the Bennettitales represent a different phylum, which branched from the cycadophyte phylum after the angiosperms, but still at the pteridostrobilus stage.
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