Journal ArticleDOI
Onset of Steroidogenesis and Differentiation of Functional LH Receptors in Rat Fetal Testicular Cultures
M.N. Gangnerau,Régine Picon +1 more
TLDR
The onset of testosterone production occurs without extrinsic stimulatory factors; (b) dcAMP initiates an early steroidogenesis; (c) the onset of functional receptors is likely free of the androgenic environment.Abstract:
The present study was performed to investigate in vitro the onset of steroidogenesis and the responsiveness to LH in rat fetal testes. The male gonads explanted on days 12.5, 13.5, and 14.5 of gestation in M199 produced testosterone from 15.5 days as is the case in vivo dcAMP (1 mM) induced an anticipated steroidogenesis on day 14.5 with secretions of testosterone (0.026 +/- 0.003 ng/gonad/24 h) and progesterone (0.078 +/- 0.005 ng/gonad/24 h), whereas LH (100 ng/ml) has no effect. Antiandrogens such as aminoglutethimide (2 mM), cyproterone acetate (1 microgram/ml), and hydroxyflutamide (1 microgram/ml) could not delay the responsiveness to LH on day 15.5. An anticipated production of testosterone on day 14.5 in presence of DHA (200 ng/ml) could not induce functional LH receptors. It would appear that: (a) the onset of testosterone production occurs without extrinsic stimulatory factors; (b) dcAMP initiates an early steroidogenesis; (c) the onset of functional receptors is likely free of the androgenic environment.read more
Citations
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Journal ArticleDOI
Origin, differentiation and regulation of fetal and adult Leydig cells.
Journal ArticleDOI
Sexual differentiation of monoaminergic neurons - genetic or epigenetic?
Ingrid Reisert,Christoph Pilgrim +1 more
TL;DR: There are a number of observations that are not explainable by the 'androgen theory of sexual differentiation' and results obtained from cultures of embryonic rat brain tissue appear to indicate that dopaminergic neurons may develop morphological and functional sex differences in the absence of sex steroids.
Journal ArticleDOI
Morphological and functional maturation of Leydig cells: from rodent models to primates
Katja J. Teerds,Ilpo Huhtaniemi +1 more
TL;DR: An updated comparative analysis of the functional and morphological maturation of LC in model species with special reference to rodents and primates finds that both in primates and rodents the formation of the mature and functionally active ALC population is critically dependent on the pituitary gonadotrophin, LH.
Journal ArticleDOI
Origin, proliferation and differentiation of Leydig cells.
TL;DR: In the absence of testes, and therefore in the presence of both AMH and testosterone, the Wolffian ducts regress, creating a permissive environment for the differentiation of the Mulleria ducts and, thereby, female reproductive organs.
Journal ArticleDOI
Prenatal testosterone and luteinizing hormone levels in male rats exposed during pregnancy to 2,3,7,8-tetrachlorodibenzo-p-dioxin and diethylstilbestrol.
Tapio Haavisto,Katri Nurmela,Raimo Pohjanvirta,Hannele Huuskonen,Faraj El-Gehani,Jorma Paranko +5 more
TL;DR: It was found that TCDD has a stimulatory effect on testicular T synthesis in the H/W fetuses and that their circulating T concentrations increased significantly, while DES, on the contrary, apparently impairs testicular steroidogenesis and pituitary function.
References
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Book ChapterDOI
Aspects of androgen-dependent events as studied by antiandrogens.
TL;DR: The chapter focuses on processes of differentiation, studies on the feedback mechanism, and libido and testicular function, and the processes of spermatogenesis that are FSH-dependent.
Journal ArticleDOI
Modifiers of steroid-hormone metabolism: a review of their chemistry, biochemistry and clinical applications
TL;DR: In vitro and in vivo methods are described that are used in the investigation of the effects of drugs on the biosynthetic pathways of steroid hormones from cholesterol and the histological effects on mitochondria and endoplasmic reticulum with their inhibitory action on enzymes associated with these sub-cellular sites.
Journal ArticleDOI
Estrogen and Androgen Receptors in Sertoli, Leydig, Myoid, and Epithelial Cells: Effects of Time in Culture and Cell Density*
TL;DR: It is concluded that androgen receptors are present in all of the testicular somatic cell lines examined and in primary Sertoli cells; estrogen receptors arepresent in Sertolis cell and Leydig cell lines, but not in myoid and endothelial cell lines; the low estrogen receptor concentration in SERToli cells cultured for 3 days increases 4-fold after 15 days in culture; and cell density is a major regulator of the concentrations of estrogen and androgen receptor in the Sert
Journal ArticleDOI
Testosterone secretion by foetal rat testes in vitro.
TL;DR: The results suggest that the functional differentiation of the testis is independent of stimulatory factors like gonadotrophins and Dibutyryl cyclic AMP was found to stimulate testosterone production significantly from 14 1/2 days of gestation onwards.
Journal ArticleDOI
Receptor binding and biological activity of steroidal and nonsteroidal antiandrogens
TL;DR: It is reported for the first time the existence of partial agonist (androgenic) activity in a number of novel antiandrogens under conditions designed to distinguish between agonists and antagonists.
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