Journal ArticleDOI
Optimization of conditions in on-line comprehensive two-dimensional reversed phase liquid chromatography. Experimental comparison with one-dimensional reversed phase liquid chromatography for the separation of peptides
TLDR
A procedure for RPLC×RPLC separations able to define, for a given analysis time, the optimized LC×LC parameters for achieving the best compromise between high peak capacity and low dilution is proposed.About:
This article is published in Journal of Chromatography A.The article was published on 2015-11-20. It has received 59 citations till now.read more
Citations
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Journal ArticleDOI
Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical Applications.
TL;DR: There simply is not enough room in LC chromatograms to separate very many compounds that behave "statistically" and the attainable peak capacity does not suffice to separate complex samples, so LC cannot easily deal with complex mixtures that contain more than a few dozen analytes.
Journal ArticleDOI
Two-Dimensional Liquid Chromatography: A State of the Art Tutorial
Dwight R. Stoll,Peter W. Carr +1 more
TL;DR: This tutorial discusses the motivations for doing two-dimensional liquid chromatography and describes the commonly used implementations of the method, and discusses the state of the art in 2D-LC performance as measured by peak capacity.
Journal ArticleDOI
Optimizing separations in online comprehensive two-dimensional liquid chromatography
TL;DR: The rationale and principles of two‐dimensional liquid chromatography experiments are summarized, advantages and disadvantages of combining different selectivities are described, and strategies to improve the quality of two-dimensional liquid Chromatography separations are discussed.
Journal ArticleDOI
Two-dimensional liquid chromatography in pharmaceutical analysis. Instrumental aspects, trends and applications.
Marion Iguiniz,Sabine Heinisch +1 more
TL;DR: The objective of this review is to give an overview of past, current and emerging trends in 2D‐LC techniques applied to pharmaceutical compounds to predict future advances in two‐dimensional separation techniqes for pharmaceutical analysis.
Journal ArticleDOI
Characterization of therapeutic antibodies and related products by two-dimensional liquid chromatography coupled with UV absorbance and mass spectrometric detection
TL;DR: Although the future is bright for further development in this area, some challenges have already been addressed through new 2D-LC methods, and it is fully expect that in the near future this list will be expanded, and that continued development will lead to methods with further improved performance metrics.
References
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Journal ArticleDOI
Gradient elution in high-performance liquid chromatography
L.R. Snyder,J.W. Dolan,J.R. Gant +2 more
TL;DR: In this article, a general theory of separation for gradient elution with reversed-phase systems is presented, and expressions for retention, resolution, band width and other separation parameters are presented as a function of experimental variables.
Journal ArticleDOI
Effect of Sampling Rate on Resolution in Comprehensive Two-Dimensional Liquid Chromatography
TL;DR: This paper studies the theoretical and experimental aspects of this sampling process of “comprehensive” two-dimensional liquid chromatography, where the column effluent from the first separation system is sequentially sampled by the second dimension separation system.
Journal ArticleDOI
Ultrahigh-Pressure Reversed-Phase Capillary Liquid Chromatography: Isocratic and Gradient Elution Using Columns Packed with 1.0-μm Particles
TL;DR: An electrically driven constant-flow syringe pump capable of generating mobile-phase pressures as high as 9000 bar (130,000 psi) is described, used in conjunction with an exponential dilution method for the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0-micron particles.
Journal ArticleDOI
Some reflections on speed and efficiency of modern chromatographic methods
TL;DR: In this article, the authors compare various techniques in terms of attainable efficiency and speed as well as of the demands on the detectors, and some speculations on future proliferation will be given.
Journal ArticleDOI
Theory of peak capacity in gradient elution.
TL;DR: The theory of peak capacity for the standard operating conditions of reversed-phase and ion-exchange chromatography is outlined and bandspreading phenomena in gradient chromatography are analyzed.
Related Papers (5)
Effect of Sampling Rate on Resolution in Comprehensive Two-Dimensional Liquid Chromatography
Effect of first-dimension undersampling on effective peak capacity in comprehensive two-dimensional separations.
Two-Dimensional Liquid Chromatography: A State of the Art Tutorial
Dwight R. Stoll,Peter W. Carr +1 more