Journal ArticleDOI
Optimizing xylanase production by a newly isolated strain CAU44 of the thermophile Thermomyces lanuginosus
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Thermomyces lanuginosus CAU44, a newly isolated thermophilic fungus strain, was used for the production of extracellular xylanase on various lignocellulosic materials under shake flask conditions and the titre of xylan enzyme activity obtained was the highest ever reported.Abstract:
Thermomyces lanuginosus CAU44, a newly isolated thermophilic fungus strain, was used for the production of extracellular xylanase on various lignocellulosic materials under shake flask conditions. High-level production of xylanase by the strain was enhanced by optimizing the type of carbon sources, substrate concentration, particle size and surfactants in the culture medium. The titre of xylanase activity obtained of up to 4156 U ml−1 was the highest ever reported.read more
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Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154
TL;DR: HPLC/RID and ESI/MS analysis of XOS mixture and purified fractions showed that XOS was a mixture of neutral oligosaccharides of DP, 2-7.9% with 73.5% xylobiose.
Journal ArticleDOI
Corncob-Induced endo-1,4-β-d-Xylanase of Aspergillus oryzae MTCC 5154: Production and Characterization of Xylobiose from Glucuronoxylan
TL;DR: Eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (XOS) production to study the effect of OSX, BWX, and raw corncob on the induction of endoxylanase in A. oryzae.
Journal ArticleDOI
Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application
Jagruti J. Pandya,Akshaya Gupte +1 more
TL;DR: Among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production, and Dilute alkali and acid-pretreated wheat straw yielded 688 and 543 mg/g reducing sugar, respectively.
Journal ArticleDOI
Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris
TL;DR: Achieving functional protein expression facilitates future efforts to optimise the cultivation conditions for heterologous xylanase production and improves the chances of obtaining functional recombinant expression.
Journal ArticleDOI
Enzyme production by industrially relevant fungi cultured on coproduct from corn dry grind ethanol plants.
Eduardo Ximenes,Bruce S. Dien,Michael R. Ladisch,Nathan S. Mosier,Michael A. Cotta,Xin-Liang Li +5 more
TL;DR: Two filamentous fungi, noted for their high cellulolytic and hemicellulolytic enzyme titers, were grown on DDGS: Trichoderma reesei Rut C-30 and Asper gillus niger NRRL 2001.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Interlaboratory testing of methods for assay of xylanase activity
TL;DR: In this paper, a collaborative investigation of assays for endo-1,4-β-xylanase activity based on production of reducing sugars from polymeric 4-O-methyl glucuronoxylan was conducted.
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Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications.
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Thermophilic Fungi: Their Physiology and Enzymes
TL;DR: This review, for the first time, compiles information on the physiology and enzymes of thermophilic fungi and indicates that eukaryotic thermophily involves several mechanisms of stabilization of enzymes or optimization of their activity, with different mechanisms operating for different enzymes.