Journal ArticleDOI
Organellar segregation, rearrangement and recombination in protoplast fusion-derived Brassica oleracea calli.
T. W. Walters,Elizabeth D. Earle +1 more
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Cauliflower protoplasts were fused to determine the effect of protoplast source and pretreatment on organellar segregation in fusion products, and 83 showed evidence of mitochondrial recombination, most commonly by formation of a novel PstI fragment near the atp9 region.Abstract:
Cauliflower protoplasts were fused to determine the effect of protoplast source and pretreatment on organellar segregation in fusion products. Mitochondrial and chloroplast type were determined for over 250 calli from eight fusions between iodoacetate-treated or γ-irradiated leaf or hypocotyl protoplasts with fertile or Ogura cytoplasms. Organelles in fusion-derived calli were identified with five mitochondrial probes and one chloroplast probe. Mitochondrial and chloroplast segregation were independent but biased. Most calli had B. oleracea chloroplasts, but more calli had Ogura mitochondria than B. oleracea ones. Neither protoplast source nor pretreatment alone affected organelle segregation. However, iodoacetate treatment of hypocotyl protoplasts reduced their mitochondrial contribution to the fusion products although it did not affect chloroplast segregation. Over half of the calli had mitochondrial genomes distinct from those of either fusion partner; many of these contained the complete mitochondrial genome of one partner along with some mitochondrial DNA from the other. Out of 258 calli, 83 showed evidence of mitochondrial recombination, most commonly by formation of a novel 11-kb PstI fragment near the atp9 region.read more
Citations
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Journal Article
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoïd protein electrophoresis.
Journal ArticleDOI
An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum x L. pennellii F2 populations
J.P.W. Haanstra,C. Wye,H. Verbakel,Fien Meijer-Dekens,P. van den Berg,P. Odinot,A.W. van Heusden,Steve Tanksley,Pim Lindhout,J. Peleman +9 more
TL;DR: Two independent F2 populations of Lycopersicon esculentum×L.
Book ChapterDOI
Brassica and Its Close Allies: Cytogenetics and Evolution
Book ChapterDOI
The Use of Cytoplasmic Male Sterility for Hybrid Seed Production
TL;DR: The use of CMS to produce hybrid seed is very cost effective and has been widely exploited in a plethora of agronomic and horticultural crops.
Journal ArticleDOI
Direct transfer of a cold-tolerant Ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion
TL;DR: The authors' procedures permit the transfer of a desirable male-sterile cytoplasm into cabbage much more rapidly than conventional backcrossing procedures.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A plant DNA minipreparation: Version II
TL;DR: The topic of this report is rap,d m,croscale methods for,solat,on of plant D N A without tile use of ul tracentr ,fugatlon wEth CsCI, which is of moderately high molecular weight and serves as a satisfactory substrate for most restrlctum cndonucleases and is statable for genom,c blot analys,s.
Journal ArticleDOI
Plant mitochondrial DNA evolved rapidly in structure, but slowly in sequence
TL;DR: The tempo and mode of mitochondrial DNA evolution in six species of crucifers from two genera, Brassica and Raphanus, are examined, finding the rate of rearrangements is extraordinarily faster in plant mtDNA than in cpDNA and animal mtDNA.
Journal ArticleDOI
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoid protein electrophoresis.
Book ChapterDOI
Isolation and structural analysis of chloroplast DNA
TL;DR: This chapter describes a filter hybridization approach for mapping chloroplast DNA (cpDNA) restriction sites along with several strategies for generating restriction fragments for use as hybridization probes.
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