Journal ArticleDOI
Orientation control of fluorescence resonance energy transfer using DNA as a helical scaffold.
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TLDR
The experimental data are found to be in excellent accord with an oriented dipole model, rather than with the averaged dipoles model conventionally assumed for FRET.Abstract:
The efficiency of fluorescence resonance energy transfer (FRET) between two chromophores positioned at opposite ends of DNA base pair domains has been investigated. The base pair domain serves as a helical scaffold which defines both the distance between chromophores and the dihedral angle between their electronic transition dipole moments, each incremental base pair increasing the distance and stepping the dihedral angle. Fluorescence quantum yields and lifetimes have been determined for both the donor and acceptor chromophores. The experimental data are found to be in excellent accord with an oriented dipole model, rather than with the averaged dipole model conventionally assumed for FRET.read more
Citations
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Materials for Fluorescence Resonance Energy Transfer Analysis: Beyond Traditional Donor-Acceptor Combinations
TL;DR: This review gives a critical overview of the major classes of fluorophore materials that may act as donor, acceptor, or both in a FRET configuration and focuses on the benefits and limitations of these materials and their combinations, as well as the available methods of bioconjugation.
Journal ArticleDOI
Artificial light-harvesting antennae: electronic energy transfer by way of molecular funnels
Raymond Ziessel,Anthony Harriman +1 more
TL;DR: Recent developments in the field of electronic energy transfer are highlighted in terms of synthesis and subsequent spectroscopic exploration.
Journal ArticleDOI
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids
Asif Iqbal,Sinan Arslan,Burak Okumus,Timothy J. Wilson,Gerard Giraud,David Norman,Taekjip Ha,David M.J. Lilley +7 more
TL;DR: It is found that the efficiency of fluorescence resonance energy transfer between Cy3 and Cy5 terminally attached to the 5′ ends of a DNA duplex is significantly affected by the relative orientation of the two fluorophores.
Journal ArticleDOI
Fluorescent nucleic acid base analogues
TL;DR: This review describes the different classes of fluorophores that can be used for studying nucleic acid-containing systems and describes the characteristics of the large group of base analogues that has an emission that is sensitive to the surrounding microenvironment.
References
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Journal ArticleDOI
The renaissance of fluorescence resonance energy transfer
TL;DR: Improvements in fluorescence resonance energy transfer have led to qualitative and quantitative improvements in the technique, including increased spatial resolution, distance range, and sensitivity.
Journal ArticleDOI
the tyranny of the lattice...
TL;DR: A systematic comparison of crystal structures of nine different B-DNA dodecamers, in three different space groups, with and without A-tracts, shows that crystal packing or lattice forces are of secondary importance for helix axis bending, minor-groove width, and propeller twist.
Journal ArticleDOI
Electronic Absorption Spectra of Neutral Perylene (C20H12), Terrylene (C30H16), and Quaterrylene (C40H20) and Their Positive and Negative Ions: Ne Matrix-Isolation Spectroscopy and Time-Dependent Density Functional Theory Calculations
Thomas M. Halasinski,Jennifer L. Weisman,R. Ruiterkamp,Timothy J. Lee,Farid Salama,Martin Head-Gordon +5 more
TL;DR: In this paper, a full experimental and theoretical study of an interesting series of polycyclic aromatic hydrocarbons, the oligorylenes, is presented, where the absorption spectra of perylene, terrylene and quaterrylene are obtained by matrix-isolation spectroscopy in Ne.
Journal ArticleDOI
Intramolecular energy hopping and energy trapping in polyphenylene dendrimers with multiple peryleneimide donor chromophores and a terryleneimide acceptor trap chromophore.
Michael Maus,Rina De,Marc Lor,Tania Weil,Sivaprasad Mitra,Uwe-M. Wiesler,Andreas Herrmann,Johan Hofkens,Tom Vosch,Klaus Müllen,Frans C. De Schryver +10 more
TL;DR: Molecular- modeling-based structures tentatively indicate that the rotation of the terryleneimide acceptor group can lead to a much smaller distance to a single donor chromophore, which could explain the occurrence of two energy-trapping rate constants.