OsNAC2 integrates auxin and cytokinin pathways to modulate rice root development
Chanjuan Mao,Chanjuan Mao,Jianmei He,Lina Liu,Qiming Deng,Xue-Feng Yao,Chun-Ming Liu,Yongli Qiao,Peng Li,Feng Ming,Feng Ming +10 more
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TLDR
It is described that OsNAC2 functions as an upstream integrator of auxin and cytokinin signals that affect CRL and CDK production to regulate cell division during root development.Abstract:
The rice root system is important for growth The crosstalk between auxin and cytokinin mediates root initiation and elongation However, it remains unclear how the transcriptional network upstream of the auxin and cytokinin signalling pathways determines root development Here, we observed that the knockdown of OsNAC2, which encodes a NAC transcription factor, increased the primary root length and the number of crown roots OsNAC2 predominantly expressed in primary root tips, crown roots and lateral root primordia, implying it influences root development Molecular analyses revealed that the expressions of auxin- and cytokinin-responsive genes were affected in OsNAC2-overexpressing (OsNAC2-OX; ON7 and ON11), RNA interference (OsNAC2-RNAi; RNAi25 and RNAi31) and CRISPR/Cas9 plants Additionally, OsNAC2 can directly bind to the promoters of IAA inactivation-related genes (GH36 and GH38), an IAA signalling-related gene (OsARF25), and a cytokinin oxidase gene (OsCKX4) Furthermore, genetic analysis of ON11/osgh36 and RNAi31/osckx4 homozygote confirmed that OsCKX4 and OsGH36 functioned downstream of OsNAC2 The mRNA levels of CROWN ROOTLESS (CRL) genes and cyclin-dependent protein kinase (CDK) genes increased in OsNAC2-RNAi and OsNAC2-cas9 lines while reduced in OsNAC2-OX lines Thus, we describe that OsNAC2 functions as an upstream integrator of auxin and cytokinin signals that affect CRL and CDK production to regulate cell division during root development This novel auxin-OsNAC2-cytokinin model should provide a new insight into the understanding of NAC TFs and crosstalk of auxin and cytokinin pathway, and can be potentially applied in agriculture to enhance rice yields by genetic approachesread more
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References
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Journal ArticleDOI
Integrative genomics viewer
James T. Robinson,Helga Thorvaldsdottir,Wendy Winckler,Mitchell Guttman,Eric S. Lander,Eric S. Lander,Gad Getz,Jill P. Mesirov +7 more
TL;DR: In this article, the authors present an approach for efficient and intuitive visualization tools able to scale to very large data sets and to flexibly integrate multiple data types, including clinical data.
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Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity
TL;DR: The results are consistent with the hypothesis that cytokinins have central, but opposite, regulatory functions in root and shoot meristems and indicate that a fine-tuned control of catabolism plays an important role in ensuring the proper regulation of cytokinin functions.
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Transcription switches for protoxylem and metaxylem vessel formation
Minoru Kubo,Makiko Udagawa,Nobuyuki Nishikubo,Gorou Horiguchi,Masatoshi Yamaguchi,Jun Ito,Tetsuro Mimura,Hiroo Fukuda,Taku Demura +8 more
TL;DR: Findings suggest that VND6 and VND7 genes are transcription switches for plant metaxylem and protoxylem vessel formation.
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Characterization of an Arabidopsis Enzyme Family That Conjugates Amino Acids to Indole-3-Acetic Acid
Paul E. Staswick,Bogdan Serban,Martha Rowe,Iskender Tiryaki,Marien T. Maldonado,Mitsa C. Maldonado,Walter P. Suza +6 more
TL;DR: Evidence is established that several GH3 genes encode IAA-amido synthetases, which help to maintain auxin homeostasis by conjugating excess IAA to amino acids.
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Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
TL;DR: NAC1, a new member of the NAC family, is induced by auxin and mediates auxin signaling to promote lateral root development and can restore lateral root formation in the auxin-response mutant tir1, indicating that NAC1 acts downstream of TIR1.