Overexpression of a Fragaria vesca MYB Transcription Factor Gene (FvMYB82) Increases Salt and Cold Tolerance in Arabidopsis thaliana
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In this article , a new MYB transcription factor (TF) family was cloned from Fragaria vesca (a diploid strawberry) and named FvMYB82.Abstract:
The MYB transcription factor (TF) family is one of the largest transcription families in plants, which is widely involved in the responses to different abiotic stresses, such as salt, cold, and drought. In the present study, a new MYB TF gene was cloned from Fragaria vesca (a diploid strawberry) and named FvMYB82. The open reading frame (ORF) of FvMYB82 was found to be 960 bp, encoding 319 amino acids. Sequence alignment results and predictions of the protein structure indicated that the FvMYB82 contained the conserved R2R3-MYB domain. Subcellular localization analysis showed that FvMYB82 was localized onto the nucleus. Furthermore, the qPCR showed that the expression level of FvMYB82 was higher in new leaves and roots than in mature leaves and stems. When dealing with different stresses, the expression level of FvMYB82 in F. vesca seedlings changed markedly, especially for salt and cold stress. When FvMYB82 was introduced into Arabidopsis thaliana, the tolerances to salt and cold stress of FvMYB82-OE A. thaliana were greatly improved. When dealt with salt and cold treatments, compared with wild-type and unloaded line (UL) A. thaliana, the transgenic lines had higher contents of proline and chlorophyll, as well as higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). However, the transgenic A. thaliana had lower level of malondialdehyde (MDA) and electrolytic leakage (EL) than wild-type and UL A. thaliana under salt and cold stress. Meanwhile, FvMYB82 can also regulate the expression of downstream genes associated with salt stress (AtSnRK2.4, AtSnRK2.6, AtKUP6, and AtNCED3) and cold stress (AtCBF1, AtCBF2, AtCOR15a, and AtCOR78). Therefore, these results indicated that FvMYB82 probably plays an important role in the response to salt and cold stresses in A. thaliana by regulating downstream related genes.read more
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Isolation and preliminary functional analysis of FvICE1, involved in cold and drought tolerance in Fragaria vesca through overexpression and CRISPR/Cas9 technologies.
Jiaxin Han,Xingguo Li,Wenhui Li,Qianqian Yang,Zhenghao Li,Zhen Chen,Long Lv,X. Y. Zhang,Deguo Han +8 more
TL;DR: In this paper , the FvICE1 was isolated from Fragaria vesca 'Hawaii 4', a bioinformatics analysis was conducted, overexpression vector and CRISPR/cas9 vector were constructed.
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MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis
TL;DR: In this paper , a new MYB (v-myb avian myeloblastosis viral)TF gene, MbMYBC1, was isolated from the M. baccata and located in nucleus.
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MbICE1 Confers Drought and Cold Tolerance through Up-Regulating Antioxidant Capacity and Stress-Resistant Genes in Arabidopsis thaliana
Yadong Duan,Jiaxin Han,B. Guo,Wenbo Zhao,Shuang Zhou,Chunwei Zhou,Lei Zhang,Xingguo Li,Deguo Han +8 more
TL;DR: In this paper , the function of drought-and cold-induced MbICE1 of Malus baccata was investigated in Arabidopsis, showing enhanced tolerance to drought and cold stresses.
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Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress
TL;DR: Wang et al. as mentioned in this paper used Shannong No.1 experimental material to simulate higher salt concentration in ginger and analyzed the physiological responses of different parts of ginger seedlings under salt stress, which led to a significant decrease in fresh and dry weight of ginger, lipid membrane peroxidation, increased sodium ion content and enhanced activity of antioxidant enzymes.
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Overexpression of a Fragaria vesca 1R-MYB Transcription Factor Gene (FvMYB114) Increases Salt and Cold Tolerance in Arabidopsis thaliana
TL;DR: In this article , a new 1R-MYB transcription factor was obtained from Fragaria vesca (a diploid strawberry) by cloning technology and given a new name, FvMYB114.
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