Journal ArticleDOI
Paradoxical shortening of scrapie incubation times by expression of prion protein transgenes derived from long incubation period mice
David Westaway,Carol Mirenda,Dallas Foster,Yeganeh Zebarjadian,Michael R. Scott,Marilyn Torchia,Shu-Lian Yang,Hana Serban,Stephen J. DeArmond,Christine Ebeling,Stanley B. Prusiner,George A. Carlson +11 more
TLDR
In this paper, the authors analyzed the effect of the ILnJ Prn-p b allele and a distinct incubation time locus designated as PRn-i on scrapie incubation times.About:
This article is published in Neuron.The article was published on 1991-07-01. It has received 125 citations till now. The article focuses on the topics: Incubation & Incubation period.read more
Citations
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Journal ArticleDOI
Molecular biology of prion diseases
TL;DR: Understanding prion diseases may advance investigations of other neurodegenerative disorders and of the processes by which neurons differentiate, function for decades, and then grow senescent.
Journal ArticleDOI
Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein
Hansruedi Büeler,Marek Fischer,Yolande Lang,Yolande Lang,Horst Bluethmann,Horst Bluethmann,Hans-Peter Lipp,Stephen J. DeArmond,Stephen J. DeArmond,Stanley B. Prusiner,Stanley B. Prusiner,Michel Aguet,Charles Weissmann +12 more
TL;DR: It is now feasible to determine whether mice devoid of PrPc can propagate prions and are susceptible to scrapie pathogenesis.
Journal ArticleDOI
Physiology of the Prion Protein
Rafael Linden,Vilma R. Martins,Marco A. M. Prado,Martín Cammarota,Ivan Izquierdo,Ricardo R. Brentani +5 more
TL;DR: A unified view of functional properties of PrP(C) indicates that the prion protein is a dynamic cell surface platform for the assembly of signaling modules, based on which selective interactions with many ligands and transmembrane signaling pathways translate into wide-range consequences upon both physiology and behavior.
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129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally normal.
TL;DR: The use of a different targeting strategy is reported, to produce inbred mice with a complete absence of both PrP protein and mRNA sequences, which are being used in experiments designed to address the role of PrP in the pathogenesis of scrapie and the replication of infectivity.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
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Novel proteinaceous infectious particles cause scrapie
TL;DR: A new term "prion" is proposed to denote a small proteinaceous infectious particle which is resistant to inactivation by most procedures that modify nucleic acids.
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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer
TL;DR: The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.