PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii.
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TLDR
The REP-PCR technique is a useful and expeditious method for the epidemiologic characterization of A. baumannii nosocomial outbreaks, the results being comparable to those obtained with the PFGE technique.About:
This article is published in Clinical Microbiology and Infection.The article was published on 2000-12-01 and is currently open access. It has received 131 citations till now. The article focuses on the topics: Acinetobacter baumannii & Pulsed-field gel electrophoresis.read more
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Acinetobacter baumannii: Emergence of a Successful Pathogen
TL;DR: This review details the significant advances that have been made in understanding of this remarkable organism over the last 10 years, including current taxonomy and species identification, issues with susceptibility testing, mechanisms of antibiotic resistance, global epidemiology, clinical impact of infection, host-pathogen interactions, and infection control and therapeutic considerations.
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Horizontal Transfer of the OXA-24 Carbapenemase Gene via Outer Membrane Vesicles: a New Mechanism of Dissemination of Carbapenem Resistance Genes in Acinetobacter baumannii
Carlos Rumbo,Esteban Fernandez-Moreira,María Merino,Margarita Poza,Jose Antonio Mendez,Nelson C. Soares,Alejandro Mosquera,Fernando Chaves,Germán Bou +8 more
TL;DR: First experimental evidence that clinical isolates of A. baumannii may release outer membrane vesicles (OMVs) as a mechanism of horizontal gene transfer whereby carbapenem resistance genes are delivered to surrounding A. BaumannII bacterial isolates is presented.
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Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside
Ming-Feng Lin,Chung-Yu Lan +1 more
TL;DR: A deep understanding of the resistance mechanisms used by MDRAB can shed light on two possible strategies to combat the dissemination of antimicrobial resistance and to develop and evaluate the efficacy of new antibiotics and to understand the role of other potential alternatives in the treatment ofMDRAB infections.
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Identification of Acinetobacter Species and Genotyping of Acinetobacter baumannii by Multilocus PCR and Mass Spectrometry
Joseph A. Ecker,Christian Massire,Thomas A. Hall,Raymond Ranken,Thuy Trang D. Pennella,Cristina Ivy,Lawrence B. Blyn,Steven A. Hofstadler,Timothy P. Endy,Paul T. Scott,Luther E. Lindler,Tacita Hamilton,Charla Gaddy,Kerry Snow,Marie Pe,Joel Fishbain,David Craft,Gregory Deye,Scott Riddell,Eric Milstrey,Bruno Petruccelli,Sylvain Brisse,Vanessa Harpin,Amy Schink,David J. Ecker,Rangarajan Sampath,Mark W. Eshoo +26 more
TL;DR: Multilocus PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) is a method that uses the amplicon base compositions to genotype bacterial species and identified at the species level at least 18 Acinetobacter species.
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Surveillance Cultures and Duration of Carriage of Multidrug-Resistant Acinetobacter baumannii
Dror Marchaim,Shiri Navon-Venezia,David A. Schwartz,Jalal Tarabeia,Iris Fefer,Mitchell J. Schwaber,Yehuda Carmeli +6 more
TL;DR: Current methodology is suboptimal to detect MDR A. baumannii carriage, and the sensitivity of surveillance cultures is low, even when six different body sites are sampled.
References
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National Committee for Clinical Laboratory Standards.
TL;DR: Many members of the Academy of Pediatrics seem to be generally unaware of the fact that the Academy has participated for ten years in a very interesting and valuable organization, the National Committee for Clinical Laboratory Standards (NCCLS).
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Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
Fred C. Tenover,Robert D. Arbeit,Richard V. Goering,P. A. Mickelsen,Barbara E Murray,D. H. Persing,B. Swaminathan +6 more
TL;DR: This research presents a novel, scalable and scalable approach that allows for real-time assessment of the severity of the infection and its impact on patients’ health.
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Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features.
TL;DR: The taxonomic status of Acinetobacter spp.
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Nosocomial pneumonia in patients receiving continuous mechanical ventilation. Prospective analysis of 52 episodes with use of a protected specimen brush and quantitative culture techniques.
Jean-Yves Fagon,Jean Chastre,Y. Domart,Jean-Louis Trouillet,Josiane Pierre,Christian Darne,Claude Gibert +6 more
TL;DR: To determine prognostic and descriptive factors of ventilator-associated (V-A) pneumonia, 567 patients who had been receiving mechanical ventilation for more than 3 days in a unit were prospectively studied.