Journal ArticleDOI
Photocurable surgical tissue adhesive glues composed of photoreactive gelatin and poly(ethylene glycol) diacrylate.
Reads0
Chats0
TLDR
Results indicate that the photocurable glues developed here may serve as a biodegradable tissue adhesive glue usable in cardiovascular surgery and endoscopic surgery.Abstract:
This article presents a novel photochemically driven surgical tissue adhesive technology using photoreactive gelatins and a water-soluble difunctional macromer (poly(ethylene glycol) diacrylate: PEGDA).The gelatins were partially derivatized with photoreactive groups, such as ultraviolet light (UV)-reactive benzophenone and visible light-reactive xanthene dye (e.g., fluorescein sodium salt, eosin Y, and rose bengal). A series of the prepared photocurable tissue adhesive glues, consisting of the photoreactive gelatin, PEGDA, and a saline solution with or without ascorbic acid as a reducing agent, were viscous solutions under warming, and their effectiveness was evaluated as hemostasis- and anastomosis-aid in cardiovascular surgery. Regardless of the type of photoreactive groups, the irradiation of the photocurable tissue adhesive glues by UV or visible light within 1 min produced water-swollen gels, which had a high adhesive strength to wet collagen film. These were due to the synergistic action of photoreactive group-initiated photo-cross-linking and photograft polymerization. An increase in the irradiation time resulted in increased gel yield and reduced water swellability. A decrease in the molecular weight of PEGDA and an increase in concentration of both gelatin and PEGDA resulted in reduced water swellability and increased tensile and burst strengths of the resultant gels. In rats whose livers were injured with a trephine in laparotomy, the bleeding spots were coated with the photocurable adhesive glue and irradiated through an optical fiber. The coated solution was immediately converted to a swollen gel. The gel was tightly adhered to the liver tissue presumably by interpenetration, and concomitantly hemostasis was completed. The anastomosis treatment with the photocurable glue in the canine abdominal or thoracic aortas incised with a knife resulted in little bleeding under pulsatile flow after declamping. Histological examination showed that the glues photocured on rat liver surfaces were gradually degraded with time in vivo with infiltration of inflammatory cells and connective tissues without necrotic sign in surrounding tissue. In addition, in the laparoscopic surgery, percutaneous delivery of the glue and its in situ photogelation on rat liver surfaces were demonstrated using a specially designed fiberscope. These results indicate that the photocurable glues developed here may serve as a biodegradable tissue adhesive glue usable in cardiovascular surgery and endoscopic surgery.read more
Citations
More filters
Journal ArticleDOI
A Review: Electrospinning of Biopolymer Nanofibers and their Applications
TL;DR: An overview of applications of nanofibrous biopolymer mats created by the electrospinning process is discussed in this article, where an introduction to biopolymers and the electro spinning process are discussed.
Journal ArticleDOI
Review: photopolymerizable and degradable biomaterials for tissue engineering applications.
TL;DR: As the authors better understand biological features necessary to control cellular behavior, smarter materials are being developed that can incorporate and mimic many of these factors.
Journal ArticleDOI
Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques.
TL;DR: To design a mesoscopically ordered structure of the matrices and scaffolds composed of nano- and microscale fiber meshes for artificial and tissue-engineering devices, two new electrospinning techniques are proposed: multilayering electrospun and mixing electrosp spinning.
Book ChapterDOI
Two-photon photopolymerization and 3D lithographic microfabrication
Hong-Bo Sun,Satoshi Kawata +1 more
TL;DR: In this article, the authors give an overview of the development and current progress of femtosecond laser micro-nanofabrication based on multiphoton absorption, and particular emphasis is placed on two-photon photopolymerization.
Patent
Compositions and systems for forming crosslinked biomaterials and associated methods of preparation and use
George Y. Daniloff,Louis C. Sehl,Olof Mikael Trollsas,Jacqueline Anne Schroeder,David M. Gravett,Philip M. Toleikis +5 more
TL;DR: Crosslinkable compositions as discussed by the authors are composed of at least two biocompatible, non-immunogenic components having reactive groups thereon, with the functional groups selected so as to enable inter-reaction between the components, i.e., crosslinking.
References
More filters
Journal ArticleDOI
Determination of free amino groups in proteins by trinitrobenzenesulfonic acid
TL;DR: A sensitive method to determine the free amino groups in proteins is presented that makes use of the reagent 2,4,6-trinitrobenzenesulfonic acid and found that sodium dodecyl sulfate was bound to some of the ϵ-amino groups of lysine in bovine serum albumin, ovalbumin, and human γ-globulin and rendered the involved amino groups unreactive toward TNBS.
Journal ArticleDOI
Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromers
TL;DR: The amphiphilic nature of the macromers causes them to assume a micellar conformation, which enables them to undergo rapid photopolymerization, resulting in the formation of crosslinked gels.
Journal ArticleDOI
Strain energy density function and uniform strain hypothesis for arterial mechanics.
TL;DR: Stress distribution through the wall thickness of the canine carotid artery was analyzed on the basis of the uniform strain hypothesis in which the wall circumferential strain was assumed to be constant over the wall cross-section under physiological loading condition.
Journal ArticleDOI
A review of the genotoxicity of food, drug and cosmetic colours and other azo, triphenylmethane and xanthene dyes.
R.D. Combes,R.B. Haveland-Smith +1 more
TL;DR: It is suggested that a human cancer hazard may exist where significant quantities of finished benzidine dye samples are handled and risks from exposures to other colours and the possibility of human germ-line mutation induction by dyestuffs cannot be meaningfully assessed.
Journal ArticleDOI
Cross-linking of gelatin capsules and its relevance to their in vitro-in vivo performance
TL;DR: It is suggested that in vitro dissolution tests of hard gelatin-containing dosage forms be conducted in two stages, one in a dissolution medium without enzymes and secondly in dissolution media containing enzymes prior to in vivo evaluation, to constitute a better indication of the in vivo behavior of gelatin-encapsulated formulations.