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Journal ArticleDOI

Phylogenetic analysis of two syntrophic propionate-oxidizing bacteria in enrichments cultures

TLDR
Experiments showed that the obtained sequences were derived from the dominant propionate-oxidizing bacteria in the enrichment cultures, which are closely related to Syntrophobacter wolinii.
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This article is published in Systematic and Applied Microbiology.The article was published on 1995-01-01. It has received 72 citations till now. The article focuses on the topics: Bacteria & Sulfate-reducing bacteria.

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Citations
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Journal ArticleDOI

Energetics of syntrophic cooperation in methanogenic degradation.

TL;DR: S syntrophically fermenting bacteria synthesize ATP by substrate-level phosphorylation and reinvest part of the ATP-bound energy into reversed electron transport processes, to release the electrons at a redox level accessible by the partner bacteria and to balance their energy budget.
Journal ArticleDOI

Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO).

TL;DR: It is completely unclear how important microbial diversity is for the control of trace gas flux at the ecosystem level, and different microbial communities may be part of the reason for differences in trace gas metabolism, e.g., effects of nitrogen fertilizers on CH4 uptake by soil; decrease of CH4 production with decreasing temperature.
Journal ArticleDOI

Temperature Gradient Gel Electrophoresis Analysis of 16S rRNA from Human Fecal Samples Reveals Stable and Host-Specific Communities of Active Bacteria

TL;DR: The results indicate that the combination of cloning and TGGE analysis of 16S rDNA amplicons is a reliable approach to monitoring different microbial communities in feces.
Journal ArticleDOI

Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes

TL;DR: Six 16S rRNA-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples, indicating that normal biological variations within the fecal populations of the volunteers were determined and indicated that these variations should be considered when evaluating the effects of agents modulating the flora.
Journal ArticleDOI

Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments

TL;DR: The instantaneous and complete inhibition of H2-dependent CH4 production that is often observed upon addition of sulfate can only be explained if a comparably high sulfate reduction potential is cryptically present in the methanogenic environment.
References
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Book

Molecular Cloning: A Laboratory Manual

TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI

DNA sequencing with chain-terminating inhibitors

TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI

The neighbor-joining method: a new method for reconstructing phylogenetic trees.

TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.

16S/23S rRNA sequencing

D. J. Lane
Journal ArticleDOI

Genomic sequencing

TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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