Phytochrome E Influences Internode Elongation and Flowering Time in Arabidopsis
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TLDR
It is concluded that in conjunction with phYB and to a lesser extent with phyD, phyE functions in the regulation of the shade avoidance syndrome.Abstract:
From a screen of M2 seedlings derived from gamma-mutagenesis of seeds doubly null for phytochromes phyA and phyB, we isolated a mutant lacking phyE. The PHYE gene of the selected mutant, phyE-1, was found to contain a 1-bp deletion at a position equivalent to codon 726, which is predicted to result in a premature stop at codon 739. Immunoblot analysis showed that the phyE protein was undetectable in the phyE-1 mutant. In the phyA- and phyB-deficient background, phyE deficiency led to early flowering, elongation of internodes between adjacent rosette leaves, and reduced petiole elongation. This is a phenocopy of the response of phyA phyB seedlings to end-of-day far-red light treatments. Furthermore, a phyE deficiency attenuated the responses of phyA phyB seedlings to end-of-day far-red light treatments. Monogenic phyE mutants were indistinguishable from wild-type seedlings. However, phyB phyE double mutants flowered earlier and had longer petioles than did phyB mutants. The elongation and flowering responses conferred by phyE deficiency are typical of shade avoidance responses to the low red/far-red ratio. We conclude that in conjunction with phyB and to a lesser extent with phyD, phyE functions in the regulation of the shade avoidance syndrome.read more
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References
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Journal ArticleDOI
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.
K Edwards,C Johnstone,C Thompson +2 more
TL;DR: This work has developed a method for the rapid extraction of small amounts of plant genomic DNA suitable for PCR analysis that is applicable to a variety of plant species and has the added advantage of not requiring any phenol or chloroform extraction.
Journal ArticleDOI
A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana.
TL;DR: A differential response of the mutants to environmental factors and their subdivision into epistatic groups is discussed in relation to a causal model for floral initiation in Arabidopsis thaliana.
Journal ArticleDOI
Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout arabidopsis development
TL;DR: It is reported here that previously described hy3 mutants have mutations in the gene coding for phytochrome B (PhyB), the first mutations shown to lie in a plant photoreceptor gene.
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Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family
TL;DR: Findings indicate that the red/far light-responsive phytochrome photoreceptor system in A. thaliana, and perhaps in all higher plants, consists of a family of chromoproteins that are heterogeneous in structure and regulation.
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Genetic Control of Light-inhibited Hypocotyl Elongation in Arabidopsis thaliana (L.) Heynh
TL;DR: In this paper, a reduced sensitivity to the inhibitory action of white light on hypocotyl elongation was observed in 41 mutants induced in Arabidopsis tbaliana at 5 different loci.