scispace - formally typeset
Journal ArticleDOI

Polarization‐sensitive scanned fiber confocal microscope

Poul M. F. Nielsen, +2 more
- 01 Nov 1996 - 
- Vol. 35, Iss: 11, pp 3084-3091
TLDR
In this article, a Se´narmont polarization rotator is used to measure the intensity and polarization orientation of linearly polarized light reflected from a birefringent sample.
Abstract
We describe a polarization-sensitive fiber optic based confocal microscope. Polarization maintaining single mode fibers are used to illuminate a specimen with linearly polarized light. The orientation of the incident linearly polarized light is continuously variable using a Se´narmont polarization rotator. A polarization-sensitive light detector enables the microscope to record the intensity and polarization orientation of the reflected light. The microscope can thus function as a conventional confocal microscope as well as detect rotations of the polarization orientation of the incident light by a birefringent specimen. Lateral scanning of the microscope focal volume is achieved by moving the fiber tip in a reciprocal layout. Confocal operation of the system is demonstrated both theoretically and experimentally. The theoretical and measured responses of the microscope to a dielectric mirror, glass slide, quarter wave plate, linear polarizer, and calcite crystal are considered. Images of a birefringent specimen are presented.

read more

Citations
More filters
Patent

Confocal optical scanning system employing a fiber laser

TL;DR: In this article, a confocal optical scanning system with a flexible optical emissive fiber or fiber laser and a lasing cavity defined within the fiber is described. And the perturbation created in the cavity by the in-coupling of the signal beam (36) is detected by a transducer (46).
Journal ArticleDOI

Polarization microscopy with stellated gold nanoparticles for robust, in-situ monitoring of biomolecules

TL;DR: This work applies a combination of a new type of plasmonic nanomaterial – stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio.
Journal ArticleDOI

Axially resolved complete Mueller matrix confocal microscopy

TL;DR: A technique is introduced that is capable of obtaining complete polarization-sensitive three-dimensional images that could reveal unknown anatomical conditions of living tissue that possess polarization-dependent signatures and indicates that the confocal sectioning of the system has a degrading effect on axially resolved Mueller matrix measurements.
Patent

Method and apparatus for determining the polarization properties of light emitted, reflected or transmitted by a material using a laser scanning microscope

TL;DR: In this article, a method and apparatus for determining the polarization properties of light emitted, reflected or transmitted by a material using a laser scanning microscope with the tested material (5) being illuminated point by point with a laser beam of known polarization state.
Journal ArticleDOI

Spatial imaging identification in a fiber-bundle confocal fluorescence microspectrometer

TL;DR: In this paper, a CCD-based fiber-bundle confocal fluorescence microspectrometer exhibiting a spectrum of individual fiber spots on the CCD was developed for surface analysis.
Related Papers (5)