Journal ArticleDOI
Positional dependence of particles in microfludic impedance cytometry
Daniel Spencer,Hywel Morgan +1 more
Reads0
Chats0
TLDR
It is demonstrated that for a nominal 40 μm × 40μm channel, the impedance signal is independent of position over the majority of the channel area, but shows large experimentally verifiable variation at extreme positions.Abstract:
Single cell impedance cytometry is a label-free electrical analysis method that requires minimal sample preparation and has been used to count and discriminate cells on the basis of their impedance properties. This paper shows experimental and numerically simulated impedance signals for test particles (6 μm diameter polystyrene) flowing through a microfluidic channel. The variation of impedance signal with particle position is mapped using numerical simulation and these results match closely with experimental data. We demonstrate that for a nominal 40 μm × 40 μm channel, the impedance signal is independent of position over the majority of the channel area, but shows large experimentally verifiable variation at extreme positions. The parabolic flow profile in the channel ensures that most of the sample flows through the area of uniform signal. At high flow rates inertial focusing is observed; the particles flow in equal numbers through two equilibrium positions reducing the coefficient of variance (CV) in the impedance signals to negligible values.read more
Citations
More filters
Journal ArticleDOI
Continuous Inertial Focusing and Separation of Particles by Shape
Mahdokht Masaeli,Elodie Sollier,Hamed Amini,Wenbin Mao,Kathryn M. Camacho,Nishit Doshi,Samir Mitragotri,Alexander Alexeev,Dino Di Carlo +8 more
TL;DR: In this paper, a shape-based separation of manmade particles or biological cells in microfluidic channels is proposed. But the method is not suitable for high-purity, high-throughput shapebased separation.
Journal ArticleDOI
Advances in resistive pulse sensors: Devices bridging the void between molecular and microscopic detection
TL;DR: This review will examine the recent progress in pore-based sensing in the nanoscale range by reviewing three new types of pore sensors - in-series, parallel, and size-tunable pores - and the fundamental physical chemistry of these techniques will be reviewed.
Journal ArticleDOI
Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization
TL;DR: This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells and discusses future research opportunities from the perspectives of both technical innovation and clinical applications.
Journal ArticleDOI
An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing.
Xiaole Mao,Ahmad Nawaz,Sz-Chin Steven Lin,Michael Ian Lapsley,Yanhui Zhao,J. Philip McCoy,Wafik S. El-Deiry,Tony Jun Huang +7 more
TL;DR: The integration of on-chip 3D particle focusing with on- chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics.
Journal ArticleDOI
Characterization of subcellular morphology of single yeast cells using high frequency microfluidic impedance cytometer
TL;DR: A microfluidic single cell impedance cytometer capable of dielectric characterization of single cells at frequencies up to 500 MHz is demonstrated and potentially allows for characterization of subcellular features in addition to the properties that are visible at lower frequencies.
References
More filters
Journal ArticleDOI
Single-cell microfluidic impedance cytometry: a review
Tao Sun,Hywel Morgan +1 more
TL;DR: The underlying principles of impedance analysis of particles are described and the state-of-the-art in the field of microfluidic impedance flow cytometry is described.
Journal ArticleDOI
Particle segregation and dynamics in confined flows.
TL;DR: Nonlinearity in finite-Reynolds-number flow results in particle migration transverse to fluid streamlines, producing the well-known "tubular pinch effect" in cylindrical pipes.
Journal ArticleDOI
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
David Holmes,David M. Pettigrew,Christian H. Reccius,James D. Gwyer,Cees van Berkel,Judith A. Holloway,Donna E. Davies,Hywel Morgan +7 more
TL;DR: A microfluidic single cell impedance cytometer that performs a white blood cell differential count and showed 95% correlation against commercial blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system.
Journal ArticleDOI
Single cell dielectric spectroscopy
TL;DR: In this article, a review of single cell dielectric spectroscopy methods is presented, including ac electrokinetic methods of dielectrophoresis and electrorotation.
Journal ArticleDOI
Sheathless inertial cell ordering for extreme throughput flow cytometry
TL;DR: This approach has the potential for future applications in cost-effective hematology or rare-cell analysis platforms with extreme throughput capabilities when integrated with suitable large field-of view imaging or interrogation methods.