Journal ArticleDOI
Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins.
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TLDR
From radish (Raphanus sativus) cotyledons, an enzyme has been partly purified which synthesizes the 7-glucopyranoside of zeatin [6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine], a compound known to occur in this species.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1979-09-12. It has received 59 citations till now. The article focuses on the topics: Zeatin & Glycoside.read more
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Journal ArticleDOI
Cytokinin metabolism and action
David W. S. Mok,Machteld C. Mok +1 more
TL;DR: This review centers on cytokinin metabolism with connecting discussions on biosynthesis and signal transduction, and important findings are summarized with emphasis on metabolic enzymes and genes.
Journal ArticleDOI
Evolution of cytokinin biosynthesis and degradation
TL;DR: This review summarizes present knowledge on the enzymes that synthesize cytokinins, form cytokinin conjugates, and carry out irreversible elimination of the hormones, including their phylogenetic analysis and possible variations in different organisms.
Journal ArticleDOI
N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana.
TL;DR: Constitutive overexpression of UGT76C1 in transgenic Arabidopsis confirmed that the recombinant enzyme functioned in vivo to glucosylate cytokinin applied to the plant and the biochemical characteristics of the N-glucosyltransferases were analyzed, and highly effective inhibitors of their activities were identified.
Journal ArticleDOI
Identification and Biochemical Characterization of anArabidopsis Indole-3-acetic Acid Glucosyltransferase
Rosamond G. Jackson,Eng-Kiat Lim,Yi Li,Mariusz Kowalczyk,Göran Sandberg,Jim Hoggett,David A. Ashford,Dianna J. Bowles +7 more
TL;DR: This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester inArabidopsis.
Journal ArticleDOI
The aromatic cytokinins
TL;DR: This review attempts to put into context what is known about 9-alkyl-BAs and compares their metabolism in regard to the practical use of cytokinins in agriculture and biotechnology.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
Journal ArticleDOI
Hydrophobic effects in reversed-phase liquid chromatography
TL;DR: The role of hydrophobic effects in revered-phase liquid chromatography is examined in this article, where a new topological index, the molecular connectivity, is introduced to represent solute surface area.
Journal ArticleDOI
Regulators of Cell Division in Plant Tissues
C. W. Parker,David S. Letham +1 more
TL;DR: The principal metabolites formed from zeatin by the roots of intact Z. mays seedlings were adenosine-5′-phosphate, zeat in riboside, adenine,Adenosine and an unknown compound termed Y.mays which was isolated and identified as 9-glucosylzeatin.
Journal ArticleDOI
A cytokinin oxidase in Zea mays.
Whitty Cd,Hall Rh +1 more
TL;DR: An enzyme activity that catalyzes conversion of N6-(Δ2-isopentenyl)adenosine (i6Ado) to adenosine was detected in cultured tobacco tissue by Paces et al. (1971) and Purification and characteristics of this enzyme in corn kernels have been studied.