Journal ArticleDOI
Presence of two new fatty acid binding proteins in catfish liver.
S. M. Di Pietro,JoséA. Santomé +1 more
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TLDR
Results herein show the presence of another two FABP in which partial amino acid sequences reveal great similarity with the corresponding sequences of other already known FABPs belonging to the heart type.Abstract:
A basic fatty acid binding protein (FABP), closely related to that of chicken liver, was isolated and characterized from catfish (Rhamdia sapo) liver in a previous work. Results herein show the presence of another two FABPs in which partial amino acid sequences reveal great similarity with the corresponding sequences of other already known FABPs belonging to the heart type. The purification procedures for both proteins involve gel filtration, anion-exchange chromatography, and sodium dodecyl sulfate – polyacrylamide gel electrophoresis (as a last step). Because both FABP N-termini were blocked, they were submitted to in-gel tryptic digestion and the resulting peptides were separated by high performance liquid chromatography, and sequenced by Edman degradation. One of these proteins presented the highest identity percentage when compared with those of the human and bovine heart and bovine brain (81%), and the other when compared with those of chicken retina (75%) and mouse and bovine heart FABP (70%). The ...read more
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Journal ArticleDOI
Liver fatty acid binding protein: species variation and the accommodation of different ligands.
TL;DR: The crystal structure of rat liver fatty acid binding protein (LFABP) and an alignment of amino acid sequences of all known species have been used to demonstrate two groups or sub-classes, and differences between the A and B groups have been postulated.
Journal ArticleDOI
Isolation, amino acid sequence determination and binding properties of two fatty‐acid‐binding proteins from axolotl (Ambistoma mexicanum) liver
TL;DR: The isolation and amino acid sequence determination of two FABPs from axolotl (Ambistoma mexicanum) liver indicate that they are paralogous proteins and indicate distinct functional properties of both liver FABP types.
Journal ArticleDOI
The cytoplasmic fatty-acid-binding proteins: thirty years and counting.
TL;DR: This review provides an entry to the mammalian and nonmammalian literature through a compendium of categorized review articles (nearly 60) and indicates that this large protein family may be integral in the relationship between lipid and carbohydrate metabolism.
Journal ArticleDOI
Amino acid sequence, binding properties and evolutionary relationships of the basic liver fatty-acid-binding protein from the catfish rhamdia sapo
S. M. Di Pietro,Esteban C. Dell'Angelica,Jacques H. Veerkamp,N. Sterin-Speziale,JoséA. Santomé +4 more
TL;DR: The phylogenetic analysis suggests that basic L-FABP from catfish, chicken and iguana diverged from the mammalian protein before the fish-tetrapod divergence, thus implying that the two types are encoded by different genes.
Journal ArticleDOI
Purification, characterization, and partial amino acid sequencing of an amphibian liver fatty acid binding protein
TL;DR: Partial amino acid sequencing showed that the toad liver FABP is related to that of mammalian liver and is clearly different from the amphibian heart FABp as well as from the frogs' amphibian intestine FABPs.
References
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Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
H. Schägger,G. von Jagow +1 more
TL;DR: A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described, and the omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.
Journal ArticleDOI
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.
TL;DR: An improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins, successfully applied for theinternal sequence analysis of membrane proteins from the rat mitochondria resolved in preparative two-dimensional gel electrophoresis.
Journal ArticleDOI
Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing.
Book ChapterDOI
Lipid-Binding Proteins: A Family of Fatty Acid and Retinoid Transport Proteins
Leonard J. Banaszak,Nathan S. Winter,Zhaohui Xu,David A. Bernlohr,S. W. Cowan,Alwyn T. Jones +5 more
TL;DR: This chapter focuses on the structural analyses and comparisons between members of a multigene family of hydrophobic ligand-binding proteins and provides a detailed comparison of intra- and extracellular lipid binding proteins with known crystal structures.