Book ChapterDOI
Probable Role of Non-exosomal Extracellular Vesicles in Colorectal Cancer Metastasis to Kidney: An In Vitro Cell Line Based Study and Image Analysis
Aviral Kumar,Reetoja Nag,Satyakam Mishra,Bandaru Ramakrishna,V. V. R. Sai,Debasish Mishra +5 more
- pp 163-174
Reads0
Chats0
TLDR
It can be said that NEVs from colon carcinoma could be a metastatic mediator for human kidney cells and it is indicated that 2D compact culture in combination with inexpensive image analytics can be a potential tool in anti-metaplastic drug discovery applications.Abstract:
Metastasis of colorectal carcinoma to the kidney is a rare phenomenon and least-investigated mechanistically. Both exosomal and non-exosomal vesicles (NEVs) from tumor tissues have been proven to be important metastatic mediators. In this light, the current work focuses on the investigation of the role of NEVs obtained from colorectal cancer cell line HCT116 in developing metastatic traits in normal human embryonic kidney cell line HEK293. ECVs were isolated via filtration method from spent media of HCT116 culture. Dynamic light scattering (DLS) analysis showed ECVs which are obtained as a retentate of 220 nm filters had an average size of 147 nm and hence may be classified as non-exosomal vesicles. NEVs obtained from HCT116 spent media were poured onto compact culture plates of HEK293 cell lines. A systematic image analysis of crystal violet-stained plates was done using the snake model for segmentation by MATLAB and analysis by ImageJ. It is evident from the image analysis data that the number of disseminated cells/colony of cells was more in NEVs treated wells than that of the untreated ones. The average distance of centrifugal cell migration (analogous to invasion) was also found to be higher in case of nECV-treated HEK293 compact cultures. Although early, but in conclusion, it can be said that NEVs from colon carcinoma could be a metastatic mediator for human kidney cells. Secondly, it is indicated that 2D compact culture in combination with inexpensive image analytics can be a potential tool in anti-metaplastic drug discovery applications.read more
References
More filters
Journal ArticleDOI
Cytological features of “noninvasive follicular thyroid neoplasm with papillary-like nuclear features” and their correlation with tumor histology
Francesca Maletta,Federica Massa,Liborio Torregrossa,Eleonora Duregon,Gian Piero Casadei,Fulvio Basolo,Giovanni Tallini,Marco Volante,Yuri E. Nikiforov,Mauro Papotti +9 more
TL;DR: The data indicate that most of the NIFTP nodules yield an indeterminate cytological diagnosis in FNAB cytology and nuclear features found in cytology samples are reproducibly identified in corresponding histology samples.
Journal ArticleDOI
MYC Mediates Large Oncosome-Induced Fibroblast Reprogramming in Prostate Cancer.
Valentina R. Minciacchi,Cristiana Spinelli,Mariana Reis-Sobreiro,Lorenzo Cavallini,Lorenzo Cavallini,Sungyong You,Mandana Zandian,Xiaohong Li,Rajeev Mishra,Paola Chiarugi,Rosalyn M. Adam,Rosalyn M. Adam,Edwin M. Posadas,Giuseppe Viglietto,Michael R. Freeman,Michael R. Freeman,Emanuele Cocucci,Neil A. Bhowmick,Dolores Di Vizio,Dolores Di Vizio,Dolores Di Vizio +20 more
TL;DR: The data show that prostate cancer-derived LO powerfully promote establishment of a tumor-supportive environment by inducing a novel reprogramming of the stroma in an AKT1-dependent manner.
Journal ArticleDOI
The role of exosomes in tumor progression and metastasis (Review).
TL;DR: Exosomes are able to form a premetastatic niche and enable the escape of cancer cells from recognition by host immune cells, indicating the significance of secreted extracellular vesicles in the development and prognosis of cancer.
Journal ArticleDOI
Emerging roles of exosomes in cancer invasion and metastasis.
TL;DR: Although their content depends on tumor type and stage, CCEs merit consideration as prognostic and diagnostic markers, as vehicles for drug delivery, and as potential therapeutic targets because they could transport various oncogenic elements.
Journal ArticleDOI
Egr-1 activation by cancer-derived extracellular vesicles promotes endothelial cell migration via ERK1/2 and JNK signaling pathways.
Yae Jin Yoon,Dae-Kyum Kim,Chang Min Yoon,Jaesung Park,Yoon Keun Kim,Tae-Young Roh,Yong Song Gho +6 more
TL;DR: Evidence that early growth response-1 (Egr-1) activation in endothelial cells is involved in the angiogenic activity of colorectal cancer cell-derived EVs is provided and will improve the understanding regarding the proangiogenic activities of EVs in diverse pathological conditions.
Related Papers (5)
Establishment and characterization of a cell line HCS1220 from human liver metastasis of colon cancer
Yi Chai,Huan Wang,Fang Zhou +2 more