Production of cell wall hydrolyzing enzymes by barley aleurone layers in response to gibberellic Acid.
Lincoln Taiz,William A. Honigman +1 more
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TLDR
It is shown that gibberellic acid stimulates endo-beta-1,4-xylanase activity in isolated aleurone layers and enhances the activity of two glycosidases: beta-xylopyranosidase and alpha-arabinofuranosid enzyme.Abstract:
The cell walls of barley ( Hordeum vulgare var. Himalaya) aleurone layers undergo extensive degradation during the tissue9s response to gibberellic acid. Previous work had shown that these cell walls consist almost entirely of arabinoxylan. In this study we show that gibberellic acid stimulates endo-β-1,4-xylanase activity in isolated aleurone layers. In addition, gibberellic acid enhances the activity of two glycosidases: β-xylopyranosidase and α-arabinofuranosidase. No gibberellic acid-stimulated cellulase activity was detected. Germination studies showed a similar pattern of enzyme development in intact seeds.read more
Citations
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Journal ArticleDOI
The chemistry of texture in fruits and vegetables
TL;DR: In this article, the chemistry of the major primary cell wall components, pectins, hemicelluloses and cellulose, under conditions found during the normal handling of fruits and vegetables is discussed.
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Reserve Polysaccharides Other Than Starch in Higher Plants
H. Meier,J. S. G. Reid +1 more
TL;DR: Reserve polysaccharides are formed by plant cells at certain physiological stages of development, usually during periods of intense photosynthetic activity, and are later “digested” to deliver carbohydrate monomers which re-enter the cells’ metabolism.
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Xylan structure, microbial xylanases, and their mode of action.
TL;DR: This review covers microbial xylanases with special emphasis on studies of sub-site mapping and the industrial applications of the microbialxylanases are also discussed.
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Two barley alpha-amylase gene families are regulated differently in aleurone cells.
TL;DR: Results of primer extension experiments utilizing probes for the 5' coding regions of both types of mRNA are consistent with some of the features of these predicted structures, and there appears to be only one major type of type B gene transcribed.
References
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Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
Gibberellic Acid-Enhanced Synthesis and Release of α-Amylase and Ribonuclease by Isolated Barley and Aleurone Layers
TL;DR: Gibberellic acid enhances the synthesis of alpha-amylase in isolated aleurone layers of barley-seeds and enhances the formation of ribonuclease which increases linearly over a 48 hour period.
Book ChapterDOI
[6] New colorimetric methods of sugar analysis
TL;DR: In this article, the authors discuss the new colorimetric methods of sugar analysis, and the inclusion of new and specific reactions for monosaccharides of biological significance is emphasized.
Journal ArticleDOI
Studies on Wheat Endosperm I. Chemical Composition and Ultrastructure of the Cell Walls
TL;DR: A method is described for the isolation of wheat endosperm cell walls free from non-endospermic cell walls in a 70 % ethanol medium which prevents the loss of watersoluble polymeric components.