Journal ArticleDOI
Protein aggregation: Pathways, induction factors and analysis
TLDR
This review gives a short outline of currently discussed pathways and induction methods for protein aggregation and describes currently employed set of analytical techniques and emerging technologies for aggregate detection, characterization and quantification.About:
This article is published in Journal of Pharmaceutical Sciences.The article was published on 2009-09-01. It has received 799 citations till now. The article focuses on the topics: Protein aggregation.read more
Citations
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Critical Evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the Measurement of Nanoparticles and Protein Aggregates
TL;DR: NTA is a powerful characterization technique that complements DLS and is particularly valuable for analyzing polydisperse nanosized particles and protein aggregates.
Journal ArticleDOI
Stability of Protein Pharmaceuticals: An Update
TL;DR: This review summarizes the advances that have been made since then regarding protein stabilization and formulation and discusses the current understanding of chemical and physical instability.
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Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars
TL;DR: The current state of the art in analytical technologies to assess three characteristics of protein biopharmaceuticals that regulatory authorities have identified as being important in development strategies for biosimilars: post-translational modifications, three-dimensional structures and protein aggregation are discussed.
Journal ArticleDOI
Aggregates in monoclonal antibody manufacturing processes.
TL;DR: In this review it is analyzed how aggregates are formed during monoclonal antibody industrial production, why they have to be removed and the manufacturing process steps that are designed to either minimize or remove aggregates in the final product.
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Shaken, not stirred: mechanical stress testing of an IgG1 antibody.
TL;DR: The data showed that shaking and stirring resulted in different species of aggregates both qualitatively and quantitatively, where stirring was found more stressful than shaking on the IgG1 formulation.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Protein Misfolding, Functional Amyloid, and Human Disease
TL;DR: The relative importance of the common main-chain and side-chain interactions in determining the propensities of proteins to aggregate is discussed and some of the evidence that the oligomeric fibril precursors are the primary origins of pathological behavior is described.
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Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
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Tricine–SDS-PAGE
TL;DR: A protocol for Tricine–SDS-PAGE is described, which includes efficient methods for Coomassie blue or silver staining and electroblotting, thereby increasing the versatility of the approach.