Journal ArticleDOI
Proteoglycan synthesis by cells cultured from regions of the rabbit flexor tendon.
Jon C. Daniel,D. K. Mills +1 more
TLDR
Immunohistochemical localization utilizing monoclonal antibodies confirms the localization of chondroitin sulfate and keratan sulfate in the compressional regions and its absence in tensional areas, indicating that adult flexor tendon cells in culture continue to express their region-specific phenotypes.Abstract:
Rabbit flexor tendons have two distinct biomechanical regions: a compressional region which is characterized by chondrocyte-like cells and abundant matrix, and a tensional region which has a typical tendon morphology with elongated cells, sparse matrix and parallel bundles of collagen fibers. Tissue culture of these regions yields two distinct populations of cells. The compressional cells in vitro synthesize high molecular weight chondroitin sulfate proteoglycan, while the tensional cells synthesize a dermatan sulfate rich, low molecular weight proteoglycan. Immunohistochemical localization utilizing monoclonal antibodies confirms the localization of chondroitin sulfate and keratan sulfate in the compressional regions and its absence in tensional areas. These observations indicate that adult flexor tendon cells in culture continue to express their region-specific phenotypes.read more
Citations
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Book ChapterDOI
Structural specialization in tendons under compression.
Kathryn G. Vogel,Thomas J. Koob +1 more
TL;DR: The hypothesis that tendon fibroblasts and the extracellular matrix they produce are modulated according to their mechanical requirements is explored and the development of cartilaginous tissue in the region of tendon subjected to compressive forces is genetically programmed.
Journal ArticleDOI
The distribution of cartilage oligomeric matrix protein (COMP) in tendon and its variation with tendon site, age and load.
TL;DR: The present data suggest that COMP is synthesized in response to, and is necessary for tendon to resist, load.
Journal ArticleDOI
Proteoglycans in the compressed region of human tibialis posterior tendon and in ligaments.
TL;DR: The pattern of proteoglycan accumulation in the compressed region of tendon was basically similar for all individuals and showed no distinctive trends related to age after puberty, and the patellar tendon was like the tensional region of the tibialis posterior.
Journal ArticleDOI
Glycosaminoglycans and proteoglycans in normal mitral valve leaflets and chordae: association with regions of tensile and compressive loading.
K. Jane Grande-Allen,Anthony Calabro,Vishal Gupta,Thomas N. Wight,Vincent C. Hascall,Ivan Vesely +5 more
TL;DR: The relative amounts and distributions of these GAGs are consistent with the tensile and compressive loads that these tissues bear, and many different chondroitin/dermatan sulfate subclasses were significantly decreased with advancing age.
Journal ArticleDOI
The distal tendon of the biceps brachii. Structure and clinical correlations.
Stephan Koch,Bernhard Tillmann +1 more
TL;DR: The structure and blood supply of 42 distal biceps tendons were investigated by means of light and electron microscopy as well as by immunohistochemistry for possible structural causes for the rupture of the tendon.
References
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Journal ArticleDOI
Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan
TL;DR: The 1/20/5-D-4 monoclonal antibody appears to recognize a common determinant in their polysaccharide moieties, consistent with several biochemical analyses showing the absence of keratan sulfate in proteoglycan synthesised by this tissue.
Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans.
TL;DR: Monoclonal antibodies recognizing determinants on chondroitinase-treated proteoglycan have been used in immunohistochemical localization studies determining the differential distribution of 4- and 6-sulfated and unsulfated proteoglycans in tissue sections of cartilage and other noncartilaginous tissues.
Journal ArticleDOI
The influence of mechanical forces on the glycosaminoglycan content of the rabbit flexor digitorum profundus tendon.
TL;DR: The physical forces acting on the flexor digitorum profundus tendon of the rabbit were altered by anterior translocation of the tendon and the glycosaminoglycan (GAG) content was determined in regions of the Achilles tendon previously under tension or previously subjected to pressure.
Journal ArticleDOI
Characterization of proteoglycans from adult bovine tendon.
K G Vogel,D Heinegård +1 more
TL;DR: Proteoglycans were extracted in good yield from the proximal, fibrous portion of adult bovine tendon with 4 m guanidine HCl and appeared to be composed of two or three subpopulations as detected by agarose/polyacrylamide electrophoresis, although they could not be effectively separated from one another for individual characterization.
Journal ArticleDOI
Ultrastructural study of tension and pressure zones in a rabbit flexor tendon.
Mervyn J. Merrilees,M. H. Flint +1 more
TL;DR: The flexor digitorum profundus tendon of the rabbit hind limb is subject to tensional forces throughout most of its length but, within a localised area which is in contact with the calcaneum and talus, it is subjected to additional compressive forces.