Proteomic discovery of cellular substrates of the ClpXP protease reveals five classes of ClpX-recognition signals.
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These results represent a description of general rules governing substrate recognition by a AAA+ family ATPase and suggest strategies for regulation of protein degradation.About:
This article is published in Molecular Cell.The article was published on 2003-03-01 and is currently open access. It has received 608 citations till now. The article focuses on the topics: Protein degradation & AAA proteins.read more
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AAA+ proteins: have engine, will work.
TL;DR: The structural organization of AAA+ proteins, the conformational changes they undergo, the range of different reactions they catalyse, and the diseases associated with their dysfunction are reviewed.
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The RpoS-mediated general stress response in Escherichia coli.
TL;DR: The complex transition from exponential growth to stationary phase has been partially dissected by analyzing the induction of RpoS after specific stress treatments, and a better understanding has been led to a better, but still far from complete, understanding of how stresses lead to RPOS induction and what RPoS-dependent genes help the cell deal with the stress.
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Proteasomes and their kin: proteases in the machine age.
TL;DR: 'Chambered proteases', including the eukaryotic 26S proteasome, use the energy of ATP to drive the unfolding and translocation of a polypeptide substrate into a chamber of sequestered proteolytic active sites.
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AAA+ proteases: ATP-fueled machines of protein destruction.
Robert T. Sauer,Tania A. Baker +1 more
TL;DR: The current understanding of the molecular mechanisms of substrate recognition, adaptor function, and ATP-fueled unfolding and translocation are reviewed.
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Making the most of affinity tags.
TL;DR: This work has shown that combinatorial tagging might be the only way to harness the full potential of affinity tags in a high-throughput setting.
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Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
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A Laboratory manual
TL;DR: To develop a program to print the barcodes using two commonly uses command sets and hence evaluates their ease of use for such applications, students should be able to program dot matrix printers, by manipulating bit level information and ink jet printers using page description language, such as PCL.
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Speculations on the functions of the major heat shock and glucose-regulated proteins.
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Role of a Peptide Tagging System in Degradation of Proteins Synthesized from Damaged Messenger RNA
TL;DR: Variants of λ repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by car boxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli.