Journal ArticleDOI
Protoplast fusion: a novel approach to organelle genetics in higher plants
TLDR
The availability of somatic plant protoplasts, which can be fused together and suitably cultured to produce somatic hybrid plants, is enabling the effects of such hybrid cytoplasms to be investigated in higher plants exhibiting maternal inheritance.Abstract:
In the majority of higher plants there is maternal inheritance of cytoplasmic organelles and, as a consequence, there are few opportunities for the study of the effects on plant phenotype of having cytoplasm initially containing organelles of both parents. Now the availability of somatic plant protoplasts, which can be fused together and suitably cultured to produce somatic hybrid plants, is enabling the effects of such hybrid cytoplasms to be investigated in higher plants exhibiting maternal inheritance. A very wide range of cytoplasmic genetic diversity, including mitochondrial and chloroplast recombinants, can be produced by such somatic hybridizations, and a theoretical model is presented to show the origins of this wide range of cytoplasmic diversity. Cybrids produced by such protoplast fusions have been shown to be of importance in plant breeding especially in relation to transfer of cytoplasmic male sterility and herbicide resistance. Protoplast fusion, including the fusion of gametic and somatic protoplasts, is also enabling the study of the inheritance of cytoplasmic controlled traits in higher plants. ORGANELLE GENETICS in an historical sense hasread more
Citations
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Journal ArticleDOI
The hows and whys of cytoplasmic inheritance in seed plants
TL;DR: In this article, various mechanisms by which organelles are, or are not, transmitted among the seed plants in order that researchers directly or indirectly involved with organelle inheritance may better understand the potential and the limitations of their investigations.
Journal ArticleDOI
INVITED SPECIAL PAPER: The hows and whys of cytoplasmic inheritance in seed plants
Book
In Vitro Culture of Trees
J. M. Bonga,P. von Aderkas +1 more
TL;DR: This chapter discusses collection, Sterilization, Excision and Culture, Laboratory Organization and Equipment, and Genetic Modification, which focuses on the latter part of the 1980s.
Journal ArticleDOI
Somatic hybridization in citrus: An effective tool to facilitate variety improvement
TL;DR: Citrus somatic hybridization and cybridization via protoplast fusion has become an integral part of citrus variety improvement programs worldwide and is expected to have a positive impact on citrus cultivar improvement efforts.
References
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Journal ArticleDOI
Handbook of plant cell culture.
TL;DR: Tanggal Ketik: 19930816, rd. nrd. nDokumentalis: ju. nmu as mentioned in this paper, Catatan Umum: Vol.1: Techniques for propagation and breeding; Vol.2and3:Crop species; Vol 4.5:ornamental species.
Journal ArticleDOI
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoid protein electrophoresis.
Journal ArticleDOI
Tripartite structure of the Brassica campestris mitochondrial genome
Jeffrey D. Palmer,C. R. Shields +1 more
TL;DR: Physical mapping studies indicate a novel sequence arrangement, consisting of three circular chromosomes, for the mitochondrial genome of Brassica campestris, and postulated to interconvert with the master chromosome via a co-integration-resolution pathway mediated by reciprocal recombination within the 2-kb repeat.
Journal ArticleDOI
Chloroplast DNA Rearrangements Are More Frequent When a Large Inverted Repeat Sequence Is Lost
TL;DR: There is a relatively stable arrangement of sequences in those genomes with the inverted repeat and a much more dynamic arrangement in those that have lost it, including the possibility that the invertedrepeat may play a direct role in maintaining a conserved arrangement of chloroplast DNA sequences.
Journal ArticleDOI
Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion
TL;DR: It is reported here, however, that the mitochondrial (mt) DNAs of cybrids are different from those of the parents and from the mixture of the two.
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