Protoporphyrin IX accumulation in cells treated with 5-aminolevulinic acid : Dependence on cell density, cell size and cell cycle
Johan Emelian Moan,Øystein Bech,Jean Michel Gaullier,Trond Stokke,Harald Steen,Li Wei Ma,Kristian Berg +6 more
TLDR
Human colon adenocarcinoma cells and Chinese hamster lung fibroblasts cells were incubated with different concentrations of 5‐aminolevulinic acid (ALA), and the production of protoporphyrin IX (PpIX) was studied using several techniques.Abstract:
Human colon adenocarcinoma cells (WiDr) and Chinese hamster lung fibroblasts cells (V79) were incubated with different concentrations of 5-aminolevulinic acid (ALA), and the production of protoporphyrin IX (PpIX) was studied using several techniques. The amount of PpIX produced per cell increased with increasing ALA concentration according to different kinetics for the 2 cell lines. For both cell lines a cell density dependency of the PpIX synthesis was observed. For saturating ALA concentrations, 2-3 times more PpIX was produced per cell at a density of 5 x 10(4) than at a density of 5 x 10(3) cells/cm2. The photosensitivity of cells appeared to increase even more than the PpIX content, indicating a cooperative effect in inactivation. The PpIX production rate increased with cell size and was about 1.9 times higher for cells in the G2 + M phase than for cells in the G1 phase of the cell cycle. Neither cell size nor cell cycle distribution were significantly dependent on cell density.read more
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Review of Neurosurgical Fluorescence Imaging Methodologies
Brian W. Pogue,Summer L. Gibbs-Strauss,Pablo A. Valdés,Kimberley S. Samkoe,David W. Roberts,Keith D. Paulsen +5 more
TL;DR: Currently, optimized probe approaches are showing efficacy in clinical trials, and fully commercialized imaging systems are emerging, which will clearly help to lead adoption into neurosurgical practice.
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Cell-type Specific Protoporphyrin IX Metabolism in Human Bladder Cancer in vitro¶
TL;DR: The in vitro culture of urothelial cells and fibroblasts indicates that the most important metabolic step for PPIX accumulation in the urinary bladder is the transition from PPIX to heme, which does support the validation of photodynamic diagnosis and might also lead the way to a highly specific tumor related molecule.
Journal ArticleDOI
Photosensitization and mechanism of cytotoxicity induced by the use of ALA derivatives in photodynamic therapy.
TL;DR: ALA-PDT can be improved with the use of ALA derivatives, reducing the amount of AlA necessary to induce efficient photosensitization, and PPIX formed from either ALA or ALA-esters equally sensitize the cells to photoinactivation.
Journal ArticleDOI
Porphyrin homeostasis maintained by ABCG2 regulates self-renewal of embryonic stem cells.
Jimmy Susanto,Yu-Hsing Lin,Yun-Nan Chen,Chia-Rui Shen,Yu-Ting Yan,Sheng-Ta Tsai,Chung-Hsuan Chen,Chia-Ning Shen,Chia-Ning Shen,Chia-Ning Shen +9 more
TL;DR: It is demonstrated that ABCG2 protects ES cells from PPIX accumulation during colony expansion, and that p53 and γH2AX acts as a downstream checkpoint ofABCG2-dependent defense machinery in order to maintain the self-renewal of ES cells.
Journal ArticleDOI
Ethylene glycol and amino acid derivatives of 5-aminolevulinic acid as new photosensitizing precursors of protoporphyrin IX in cells.
TL;DR: Ethylene glycol esters and amino acid pseudodipeptide derivatives of ALA are synthesized and characterized as potential specific substrates for cellular esterases and aminopeptidases, respectively and shown to be useful at concentrations below their cytotoxicity threshold.
References
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Photodynamic Therapy
TL;DR: A comprehensive review of mechanisms of subcellular and tumor localization of photosensitizing agents, as well as of molecular, cellular, and tumor responses associated with photodynamic therapy, are discussed.
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TL;DR: In the ongoing clinical trial of ALA-induced Pp IX photodynamic therapy, the response rate for basal cell carcinomas following a single treatment has been 90% complete response and 7.5% partial response for the first 80 lesions treated.
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Endogenous protoporphyrin IX, a clinically useful photosensitizer for photodynamic therapy.
TL;DR: Preclinical studies in experimental animals and human volunteers indicate that ALA can induce a localized tissue-specific photosensitization if administered by intradermal injection, opening the possibility of using ALA-induced PpIX to treat tumors that are too thick or that lie too deep to be accessible to either topical or locally injected ALA.
Journal ArticleDOI
The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen
Johan Emelian Moan,Kristian Berg +1 more
TL;DR: NHIK 3025 cells were incubated with Photofrin II and/or tetra (3‐hydroxyphenyl)porphyrin and exposed to light at either 400 or 420 nm and the kinetics of the photodegradation of the dyes were studied.