Purification of a colony stimulating factor from culture cell lines propagated from human lung.
Adel A. Yunis,Adel A. Yunis,Grace K. Arimura,Grace K. Arimura,Ming Chi Wu,Ming Chi Wu,M A Gross,M A Gross,Yvonne Purcell,Yvonne Purcell +9 more
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TLDR
Serum-free conditioned medium prepared from these cultures provides an excellent source of CSF and propagating cell lines from autopsy lung tissue which secrete CSF into the medium overcome considerable difficulty in purification.About:
This article is published in FEBS Letters.The article was published on 1978-06-15 and is currently open access. It has received 11 citations till now.read more
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Journal ArticleDOI
Methods for the purification, assay, characterization and target cell binding of a colony stimulating factor (CSF-1)
E.R. Stanley,L.J. Guilbert +1 more
Journal ArticleDOI
Separation of functionally distinct human granulocyte-macrophage colony-stimulating factors.
TL;DR: Mixing and titration experiments indicated that the differences in functional specificities of the two GM-CSFs were not due to the presence of specific inhibitory molecules or lower absolute levels of CSF in one fraction relative to the other.
Journal ArticleDOI
The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT.
TL;DR: Subcellular fractionation of GCT cells indicates that there are pools of preformed CSAs primarily associated with the cell cytosol that have similar apparent molecular weights to their secreted counterparts.
Patent
Isolated native primate GM-CSF protein
TL;DR: In this article, a method for purifying CSF protein is described, which consists of precipitating the protein with ammonium sulfate at 80% saturation to form a pellet containing the CSF proteins; resuspending the pellet in a buffered solution at a pH in the range of about 6 to about 8.
References
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A rapid, sensitive, and specific method for the determination of protein in dilute solution
TL;DR: This protein assay is described in which the sample is precipitated with trichloroacetic acid in the presence of sodium dodecylsulfate, filtered off on a Millipore membrane and stained with Amidoschwarz 10B, and its absorbance determined at 630 nm.
Journal ArticleDOI
The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TL;DR: A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.
Journal ArticleDOI
The cloning of normal “Mast” cells in tissue culture
Dov H. Pluznik,Leo Sachs +1 more
TL;DR: The results indicate that the substance(s) required for the growth and differentiation of “mast” cells can pass through agar.
Journal ArticleDOI
Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells
TL;DR: Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures and the active factor was not dependent on the presence of adherent marrow cells with endogenous colony-stimulating activity.
Journal ArticleDOI
A low molecular weight factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro.
J. W. Sheridan,D. Metcalf +1 more
TL;DR: Medium conditioned by excised whole lungs from endotoxin‐injected C57BL mice was highly active in stimulating hemopoietic colony formation, particularly of granulocytic type, in agar cultures of mouse bone marrow cells.