Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells
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Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures and the active factor was not dependent on the presence of adherent marrow cells with endogenous colony-stimulating activity.About:
This article is published in Blood.The article was published on 1977-04-01 and is currently open access. It has received 415 citations till now. The article focuses on the topics: Sephadex & Gel electrophoresis.read more
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The hemopoietic colony stimulating factors
TL;DR: It was not until the introduction in 1966 of methods for growing colonies of normal hemopoietic cells in semisolid culture, that progress was made in identifying and characterizing corresponding specific regulators for other hemopOietic populations.
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The nature and action of granulocyte-macrophage colony stimulating factors
Antony W. Burgess,Donald Metcalf +1 more
TL;DR: Information on the regulation of GM-CSF production, on the modulators of its action on specific target cells, and on its role in vivo will be required before the physiologic function of this molecule can be properly assessed.
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The Murine Placenta Contains Hematopoietic Stem Cells within the Vascular Labyrinth Region
TL;DR: It is demonstrated here that the placenta also harbors adult-repopulating HSCs, and placental cell expression of other HSC markers and transcription factors suggests that HSC emergence may occur in the Placenta, as has been proposed for other embryonic hematopoietic sites.
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Identification of lactoferrin as the granulocyte-derived inhibitor of colony-stimulating activity production.
TL;DR: Microgram quantities of LF acted in vivo to inhibit rebound granulopoiesis and CSA production in CD1 and C57Bl/6 mice pretreated with cyclophosphamide, which strongly implicate LF as a physiological regulator of granulopsioiesis.
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Purification and some characteristics of human T-cell growth factor from phytohemagglutinin-stimulated lymphocyte-conditioned media
James W. Mier,Robert C. Gallo +1 more
TL;DR: Human T-cell growth factor, a mitogenic protein that appears in the media of cultured lymphocytes after phytohemagglutinin-stimulation, has been purified more than 400-fold from serum-free conditioned media by using a sequence of ion exchange chromatography and gel filtration, indicating that the factor is a second signal in the T- cell immune response.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TL;DR: A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.
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Human bone marrow colony growth in agar-gel.
TL;DR: A technique for growing human bone marrow cell colonies in agar-gel medium is described in this paper, where feeder layers containing 1 × 106 normal human peripheral white blood cells are used as the stimulus for colony growth.
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Colony Formation by Normal and Leukemic Human Marrow Cells in Culture: Effect of Conditioned Medium From Human Leukocytes
TL;DR: "Conditioned medium" obtained from cultures of human peripheral leukocytes promoted the growth of human marrow cells in cell culture and permitted the growthof small colonies from the marrow of patients with acute myelogenous leukemia in relapse.