Quantification and stability of human adenoviruses and polyomavirus JCPyV in wastewater matrices.
Sílvia Bofill-Mas,Nestor Albinana-Gimenez,Pilar Clemente-Casares,Ayalkibet Hundesa,Jesus Rodriguez-Manzano,Annika Allard,Miquel Calvo,Rosina Girones +7 more
TLDR
Results confirm the suitability of both viruses as indicators of human fecal viral pollution and showed high stability in urban sewage.Abstract:
Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.read more
Citations
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Molecular detection of pathogens in water – The pros and cons of molecular techniques
Rosina Girones,María Antonia Ferrús,José Luis Izquierdo Alonso,Jesus Rodriguez-Manzano,Byron Calgua,Adriana de Abreu Corrêa,Adriana de Abreu Corrêa,Ayalkibet Hundesa,Anna Carratalà,Sílvia Bofill-Mas +9 more
TL;DR: The pros and cons of molecular techniques for the detection and quantification of pathogens in water are discussed and further refinement is required in order to be standardized and applicable to a diversity of matrices.
Journal ArticleDOI
Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks
Maria Hellmér,Nicklas Paxéus,Lars O. Magnius,Lucica Enache,Birgitta Arnholm,Annette Johansson,Tomas Bergström,Helene Norder +7 more
TL;DR: The presence of eight pathogenic viruses was investigated in sewage to explore whether their identification could be used as an early warning of outbreaks and provide early warning before the causative pathogens have been recognized in health care.
Journal ArticleDOI
Raw Sewage Harbors Diverse Viral Populations
Paul G. Cantalupo,Byron Calgua,Guoyan Zhao,Ayalkibet Hundesa,Adam D. Wier,Joshua P. Katz,Michael Grabe,Roger W. Hendrix,Rosina Girones,David Wang,James M. Pipas +10 more
TL;DR: Deep sequencing nucleic acids obtained from virion populations enriched from raw sewage revealed 234 known viruses, including 17 that infect humans, making untreated wastewater the most diverse viral metagenome examined thus far.
Journal ArticleDOI
Metagenomic analysis of viruses in reclaimed water.
TL;DR: Reclaimed water contained 1000-fold more virus-like particles than potable water, and contained a wealth of novel single-stranded DNA and RNA viruses related to plant, animal and insect viruses.
Journal ArticleDOI
Virus hazards from food, water and other contaminated environments.
David Rodríguez-Lázaro,Nigel Cook,Franco Maria Ruggeri,Jane Sellwood,Abid Nasser,Maria São José Nascimento,Martin D'Agostino,Ricardo Santos,Juan-Carlos Saiz,Artur Rzeżutka,Albert Bosch,Rosina Girones,Annalaura Carducci,Michelle Muscillo,Katarina Kovač,Marta Diez-Valcarce,Apostolos Vantarakis,Carl-Henrik von Bonsdorff,Ana Maria de Roda Husman,Marta Hernández,Wim H. M. van der Poel,Wim H. M. van der Poel +21 more
TL;DR: M Molecular epidemiology and surveillance of environmental samples are necessary to elucidate the public health hazards associated with exposure to environmental viruses and the human pathogenic viruses that are most relevant in this context are nonenveloped.
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