Journal ArticleDOI
Quantitative chromatography of homologous glucose oligomers and other saccharides using polyacrylamide gel
TLDR
A rapid method for the separation and quantitation of mono- and oligosaccharides is described, based on the use of Bio-Gel P-2, minus 400 mesh, in a properly designed column with water as eluent at 65°.About:
This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 135 citations till now. The article focuses on the topics: Isomaltose & Maltose.read more
Citations
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Outer membrane of gram-negative bacteria. XII. Molecular-sieving function of cell wall.
G M Decad,H Nikaido +1 more
TL;DR: The permeability function the cell wall of gram-negative bacteria such as Salmoenlla was investigated by producing cells with an expanded periplasmic volume, and incubating them with radioactive non-utilizable oligo- and polysaccharides or polyethylene glycols, finding that only di- and trisaccharides could fully diffuse into theperiplasm, whereas higher-molecular-weight saccharides were nonpenetrable.
Journal ArticleDOI
Isolation and partial characterization of an extracellular glucansucrase from Leuconostoc mesenteroides NRRL B-1355 that synthesizes an alternating (1→6), (1→3)-α-d-glucan
Gregory L. Côté,John F. Robyt +1 more
TL;DR: The glucans were identified by physical appearance, the concentration of ethanol required for precipitation, periodate-oxidation behavior, and susceptibility to hydrolysis by endodextranase.
Journal ArticleDOI
A new substrate for investigating the specificity of β-glucan hydrolases
TL;DR: A new 1,3; 1,4-/Iglucan with these properties has been prepared and its use in studies on the specificity of /3- glucan endohydrolases is described.
Journal ArticleDOI
The distribution of fructans in onions
B. Darbyshire,Robert J Henry +1 more
TL;DR: In this article, the nonstructural carbohydrate content of individual leaf bases of onion bulbs was determined, and confirmed the presence of glucose, fructose, sucrose and a series of fructans as the only components.
Journal ArticleDOI
Production, purification, and properties of dextransucrase from Leuconostoc mesenteroides NRRL B-512F.
John F. Robyt,Timothy F. Walseth +1 more
TL;DR: The production of dextransucrase from Leuconostoc mesenteroides NRRL B-512F was stimulated 2-fold by the addition of 0.005% of calcium chloride to the medium; levansucrase levels were unaffected; and the mannoglycoprotein character of the enzyme was confirmed.
References
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Journal ArticleDOI
Chromatographic Separation of Sugars on Charcoal1
Roy L. Whistler,Donald F. Durso +1 more
TL;DR: In this paper, Coulombic energy calculations were performed to confirm the enhanced dielectric constant effect on the rate in acetone-water media were less by an accounted for the ionic strength data, but reorder of magnitude than has been observed when vealed a dielectrics constant effect which, while ethyl alcohol-water was used as a solvent.
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Preparation of Mannobiose, Mannotriose, and a New Mannotetraose from Saccharomyces cerevisiae Mannan
Yuan-Chuan Lee,Clinton E. Ballou +1 more
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The correlation between molecular weight and elution behaviour in the gel chromatography of proteins
Helmut Determann,Wolfgang Michel +1 more
TL;DR: A mathematical treatment of the Gel chromatography process, based on these empirical equations and avoiding assumptions about the microstructure of the gel, strengthens the concept of the exclusion mechanism.
Journal ArticleDOI
“Molecular-sieve” chromatography of proteins on columns of cross-linked polyacrylamide☆
Stellan Hjertén,Rolf Mosbach +1 more
TL;DR: Columns packed with cross-linked polyacrylamide have been used for chromatographic separation of high molecular weight substances, especially proteins, and there is a positive correlation between the molecular size of a protein and its Rf value.