Journal ArticleDOI
Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method
Jun Young Kim,Semi Jeon,Hogeun Rhie,Bok-Kwon Lee,Mi-Sun Park,Hoanjong Lee,Jina Lee,Seonghan Kim +7 more
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TLDR
This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type β-lactamases and it not only reduces the time for classification but also increases the accuracy for detection.Abstract:
A multiplex PCR method has been developed to classify extended spectrum β-lactamase (ESBL) and plasmidmediated AmpC β-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type β-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBLproducing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of β-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.read more
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The prevalence of multidrug-resistant Aeromonas spp. in the municipal wastewater system and their dissemination in the environment.
Monika Harnisz,Ewa Korzeniewska +1 more
TL;DR: The results of this study indicate that regardless of the applied treatment, municipal sewage may be a reservoir of antibiotic-resistant bacteria, antibiotic resistance and virulence genes and that treated water can contaminate other environmental domains.
Journal ArticleDOI
The impact of WWTP size and sampling season on the prevalence of antibiotic resistance genes in wastewater and the river system.
Monika Harnisz,Edyta Kiedrzyńska,Marcin Kiedrzyński,Ewa Korzeniewska,Małgorzata Czatzkowska,Izabela Koniuszewska,Adam Jóźwik,Sebastian Szklarek,Sebastian Niestępski,Maciej Zalewski +9 more
TL;DR: The results of statistical analyses indicate that in small WWTPs, the absolute concentration of ARGs can be predicted based on the biochemical oxygen demand, in routine water analyses, however, none of the studied parameters supported predictions of ARG abundance in medium-sized and largeWWTPs or in river water.
Journal ArticleDOI
Prevalence and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in healthy children and associated risk factors
TL;DR: High rate of colonization in healthy children with ESBL-PE was observed, regular consumption of dietary products from animal source (meat or chicken) were associated with this colonization in the community in non-hospitalized children.
Journal ArticleDOI
Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China.
TL;DR: This work provides a comprehensive analysis of PMQR genes in aquatic environments in Jinan, China, and provides information with which combat with the antimicrobial resistance problem may be fought.
Journal ArticleDOI
Extended-Spectrum Β-Lactamase and Plasmid-Mediated AMPC Genes in Swine and Ground Pork
Carla Sabia,Sara Stefani,Patrizia Messi,Simona De Niederhausern,Moreno Bondi,Carla Condò,Ramona Iseppi,Immacolata Anacarso +7 more
TL;DR: The prevalence of ESBL and AmpC-producing Enterobacteriaceae isolated from rectal swabs of healthy swine and samples of ground pork were determined and ESBL/AmpC-positive strains were confirmed by PCR and DNA sequence analysis.
References
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Journal ArticleDOI
Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat
TL;DR: β-Lactamases continue to be the leading cause of resistance to β-lactam antibiotics among gram-negative bacteria and are now found in a significant percentage of Escherichia coli and Klebsiella pneumoniae strains in certain countries.
Journal ArticleDOI
Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes.
TL;DR: They were initially reported in the second half of the 1980s, and their rate of dissemination among bacteria and in most parts of the world has increased dramatically since 1995, with the CTX-M β-lactamases being the most widespread enzymes.
Journal ArticleDOI
New β-Lactamases in Gram-Negative Bacteria: Diversity and Impact on the Selection of Antimicrobial Therapy
George M. Eliopoulos,Karen Bush +1 more
TL;DR: Of the 340 discrete beta-lactamases that have been identified, the most important groups of enzymes that are continuing to proliferate include the plasmid-encoded cephalosporinases, the metallo-beta-l lactamases, and the extended-spectrum beta- lactamase.
Journal ArticleDOI
Phenotypic and Molecular Detection of CTX-M-β-Lactamases Produced by Escherichia coli and Klebsiella spp.
TL;DR: A molecular detection assay for the identification of CTX-M-β-lactamase genes was developed and used to investigate the prevalence of these enzymes among clinical isolates of Escherichia coli and Klebsiella species in the Calgary Health Region during 2000 to 2002.
Journal ArticleDOI
Epidemiology and Clinical Features of Bloodstream Infections Caused by AmpC-Type-β-Lactamase-Producing Klebsiella pneumoniae
Hyunjoo Pai,Cheol-In Kang,Jeong Hum Byeon,Ki Deok Lee,Wan Beom Park,Hong Bin Kim,Eui Chong Kim,Eui Chong Kim,Myoung Don Oh,Kang Won Choe +9 more
TL;DR: The prevalence of AmpC enzyme-producing K. pneumoniae was high at the Seoul National University Hospital, and the clinical features and outcomes for the patients infected with AmpC-producing organisms were similar to those for the Patients infected with TEM- or SHV-related ESBL producers.