Journal ArticleDOI
Rapid isolation of genomic DNA from gram-negative bacteria.
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This article is published in Trends in Genetics.The article was published on 1992-10-01. It has received 134 citations till now. The article focuses on the topics: genomic DNA & Isolation (microbiology).read more
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Extremely rapid extraction of DNA from bacteria and yeasts.
Hai-Rong Cheng,Ning Jiang +1 more
TL;DR: A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented and the supernatant contains DNA suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction.
Journal ArticleDOI
Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis
Scott Quainoo,Jordy P. M. Coolen,Sacha A. F. T. van Hijum,Martijn A. Huynen,Willem J. G. Melchers,Willem van Schaik,Heiman F. L. Wertheim +6 more
TL;DR: This review assesses the feasibility of whole-genome sequencing (WGS) technologies and bioinformatics analysis tools for nosocomial outbreak analyses, and presents a real-world example of the implementation of WGS for antimicrobial resistance analysis.
Journal ArticleDOI
New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1.
Barbara Silakowski,Hans Ulrich Schairer,Heidi Ehret,Brigitte Kunze,Stefan Weinig,Gabriele Nordsiek,Petra Brandt,Helmut Blöcker,Gerhard Höfle,Stefan Beyer,Rolf Müller +10 more
TL;DR: The biosynthetic mta gene cluster responsible for myxothiazol formation from the fruiting body forming myxobacterium Stigmatella aurantiaca DW4/3-1 was sequenced and analyzed and provided insight into the mechanism of the formation of the terminal amide of myxOTHiazol.
Journal ArticleDOI
The Biosynthesis of the Aromatic Myxobacterial Electron Transport Inhibitor Stigmatellin Is Directed by a Novel Type of Modular Polyketide Synthase
Nikolaos Gaitatzis,Barbara Silakowski,Brigitte Kunze,Gabriele Nordsiek,Helmut Blöcker,Gerhard Höfle,Rolf Müller +6 more
TL;DR: Results of feeding experiments are in good agreement with the proposed biosynthetic scheme, and in contrast to all other PKS type I systems reported to date, each module of StiA–J is encoded on a separate gene.
Journal ArticleDOI
Steroid biosynthesis in prokaryotes: identification of myxobacterial steroids and cloning of the first bacterial 2,3(S)‐oxidosqualene cyclase from the myxobacterium Stigmatella aurantiaca
TL;DR: The cloning of the first bacterial steroid biosynthesis gene, cas, encoding the cycloartenol synthase (Cas) of S. aurantiaca is described, indicating a close evolutionary relationship between myxobacterial and eukaryotic steroids biosynthesis.
References
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A simple salting out procedure for extracting DNA from human nucleated cells
TL;DR: A rapid, safe and inexpensive method was developed to simplify the deprotein-ization procedure that yielded quantities comparable to those obtained from phenol-chloroform extractions, rendering the entire process of RFLP analysis free of toxic materials.
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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In situ transposon replacement and isolation of a spontaneous tandem genetic duplication.
Leon Avery,Dale Kaiser +1 more
TL;DR: The methods described here are applicable to any bacterium into which transposons can be introduced, and for which some means of genetic exchange is available, and were used to isolate a spontaneous tandem duplication of part of the M. xanthus chromosome.