Patent
Recombinant dna sequences, vectors containing them and method for the use thereof
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Recombinant DNA sequences which encode the complete amino acid sequence of a glutamine synthetase, vectors containing such sequences, and methods for their use, in particular as dominant selectable markers, for use in co-amplification of non-selected genes and in transforming host cell lines to glutamine independence.Abstract:
Recombinant DNA sequences which encode the complete amino acid sequence of a glutamine synthetase, vectors containing such sequences, and methods for their use, in particular as dominant selectable markers, for use in co-amplification of non-selected genes and in transforming host cell lines to glutamine independence.read more
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References
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Processes for inserting dna into eucaryotic cells and for producing proteinaceous materials
TL;DR: The insertion of DNA molecules into eucaryotic cells is accomplished by cotransforming eucarial cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker as mentioned in this paper.
Journal ArticleDOI
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Diane Pennica,William E. Holmes,William J. Kohr,Richard N. Harkins,Gordon A. Vehar,Carole A. Ward,William F. Bennett,Elizabeth Mcleod Yelverton,Peter H. Seeburg,Herbert L. Heyneker,David V. Goeddel,Desire Collen +11 more
TL;DR: Bacterial clones containing human tissue-type plasminogen activator cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells and a polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.
Patent
Method for dominant selection in eucaryotic cells
TL;DR: Recombinant DNA sequences which encode the complete amino acid sequence of a glutamine synthetase, vectors containing such sequences, and methods for their use, in particular as dominant selectable markers, for use in co-amplificiation of non-selected genes and in transforming host cell lines to glutamine independence as discussed by the authors.
Journal Article
Herbicide-resistant alfalfa cells: an example of gene amplification in plants.
TL;DR: Southern blot analysis of wild-type and variant cell DNAs using this cDNA as a probe indicate that resistance to L-phosphinothricin is a consequence of a four- to 11-fold amplification of one GS gene resulting in about an eightfold increase in mRNA levels, and an increased enzyme synthesis sufficient to overcome the toxic effects of the inhibitor.
Patent
Gene amplification in eukaryotic cells
TL;DR: In this article, a DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing an auxotrophic host.