Relatedness Among Rhizobium and Agrobacterium Species Determined by Three Methods of Nucleic Acid Hybridization
TLDR
Results indicated close relationships between R. lupini and R. japonicum, and (with less certainty) betweenR.Citations
More filters
Journal ArticleDOI
NOTES: Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a Genus of Slow-Growing, Root Nodule Bacteria from Leguminous Plants
TL;DR: The slow-growing, non-acid-producing root nodule bacteria of leguminous plants should be separated from the fast- growing, acid-producing strains and placed in a new genus, and the name proposed is Bradyrhizobium.
Journal ArticleDOI
Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris Nodules
TL;DR: Thirty-one strains of two new genomic species of rhizobia isolated from root nodules of Phaseolus vulgaris and originating from various locations in France were compared with reference strains by performing a numerical analysis of 64 phenotypic features, revealing that each genomic species formed a lineage independent of the lineages formed by the previously recognized species of Rhizobia.
Journal ArticleDOI
Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti
TL;DR: All of the ten independent spontaneous non-nodulating derivatives of three strains of R. meliloti were shown to have suffered a deletion in the nif DH region of the megaplasmid, which indicates that the same replicon carries genes controlling early and late functions in symbiosis.
Journal ArticleDOI
DNA : DNA hybridization studies of Rhizobium japonicum and related Rhizobiaceae
TL;DR: The results indicated that strains labelled as R. japonicum can be separated into at least three DNA homology groups, and reference strains of other Rhizobium species, with the exception of R. lupini, were not closely related to these homologygroups.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
The quantitative measurement of DNA hybridization from renaturation rates.
TL;DR: A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture, and the advantages are summarized.
Journal ArticleDOI
Preparation of transforming deoxyribonucleic acid by phenol treatment
Hiuga Saito,Kin-ichiro Miura +1 more
TL;DR: The final DNA preparation has high transforming activity, especially for the joint transformation of linked markers, as well as high purity and low sedimentation coefficients.