Regulation of beta-galactoside alpha 2,6-sialyltransferase gene expression by dexamethasone.
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TLDR
Exposure of H35 cells to 1 microM dexamethasone for 24 h causes a 3-4-fold enrichment of sialyltransferase mRNA and a corresponding increase in enzymatic activity, implying that different pathways exist for the induction of participants in the acute phase response.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-01-25 and is currently open access. It has received 85 citations till now. The article focuses on the topics: Sialyltransferase & Puromycin.read more
Citations
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Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation.
James C. Paulson,Karen J. Colley +1 more
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Evolutionary considerations in relating oligosaccharide diversity to biological function.
Pascal Gagneux,Ajit Varki +1 more
TL;DR: It is argued that exogenous selection pressures mediated by viral and microbial pathogens and parasites that recognize glycans have played a more prominent role, favoring intra- and interspecies diversity.
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The oligosaccharides of glycoproteins: bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties.
TL;DR: The recent data concerning the effects of bioprocess factors on the oligosaccharide structure of human therapeutic glycoproteins, with particular emphasis on the influence of the host cell are organized.
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Environmental effects on protein glycosylation.
TL;DR: This review organizes the published data from in vitro cell culture and tissue culture studies that demonstrate direct effects of the culture environment on N-linked glycosylation, including the cell culture environment.
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The molecular and cell biology of glycosyltransferases
Ralf Kleene,Eric G. Berger +1 more
References
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal ArticleDOI
Assembly of asparagine-linked oligosaccharides.
TL;DR: The structure of ASPARAGINE-LINKed OLIGOSACCI-IARIDES and transfer-Oligosaccharide Structural Requirements, and Sequence of Processing and Specificity of Processing Enzymes are presented.
Journal ArticleDOI
HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS: A Biochemical and Fine Structural Study
M. N. Berry,Daniel S. Friend +1 more
TL;DR: Cytoplasmic vacuolization in a small percentage of cells and potassium loss are the only indications of cell injury detected, and the isolated cells are comparable to normal hepatic parenchymal cells in situ in appearance and function.
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Tissue-specific expression of sialyltransferases.
Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation.
James C. Paulson,Karen J. Colley +1 more