Journal ArticleDOI
Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture
Reads0
Chats0
TLDR
It is found that there is no strict correlation between cell morphology and type of collagen synthesised in cartilage colonies kept in monolayer culture at low density.Abstract:
WHEN chondrocytes from sternal or articular cartilage are kept in monolayer culture at low density, they eventually lose their cartilage phenotype1–4. Within four passages or approximately 1 month in culture they change from a polygonal or round to a flattened, amoeboid-like shape5–7, and instead of cartilage collagen (type II collagen8) they synthesise the genetically different type I collagen. It is not known whether there is a strict correlation between the occurrence of cell flattening and the change in collagen synthesis within individual cells. We have reported that preferentially flattened, fibroblast-like cells at the edge of cartilage colonies synthesise type I collagen, whereas round or polygonal chondrocytes generally synthesise type II collagen1–3. The change is nearly complete in a culture at a time when excessive flattening is observed4. Using an immunofluorescence double staining technique9,10, we have now found that there is no strict correlation between cell morphology and type of collagen synthesised.read more
Citations
More filters
Dissertation
Spinal cord cellular response to wear debris from metal-on-metal total disc replacements
TL;DR: This study highlighted the necessity for appropriate cell culture environments in biomaterial biocompatibility testing and hypothesised that, utilising a 3D type-I collagen gel, enabling glial cells to behave in a more physiologically relevant manner, the effects of increasing concentrations of metallic wear particles on glial cell viability, cellular reactivity, and cytokine release could be more accurately determined.
Dissertation
Stem cell expansion and bioreactor development
TL;DR: This paper aims to provide a chronology of the events leading up to and including the publication of this book and some of the key events leading to its publication.
Book ChapterDOI
Materials and assay systems used for three‐dimensional cell culture
Suparna Sanyal,Marshall Kosovsky +1 more
Journal ArticleDOI
Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration
TL;DR: In this paper, quercetin (QUE) modified PHBV fibrous scaffolds were prepared by a two-step surface modification method to facilitate the growth of chondrocytes.
Book ChapterDOI
Chapter 1 – Stem cells
J.M. Jukes,Sanne K. Both,Janine N. Post,Clemens van Blitterswijk,Hermanus Bernardus Johannes Karperien,Jan de Boer +5 more
TL;DR: This chapter defines stem cells and their properties and identifies the major differences between embryonic and adult stem cells.
References
More filters
Journal ArticleDOI
Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo.
H von der Mark,K von der Mark +1 more
TL;DR: Specific antibodies against skin and bone collagen and cartilage collagen are prepared for the study of differential collagen synthesis during development of the chick embryo by immunofluorescence.
Journal ArticleDOI
Changes in type of collagen synthesized as clones of chick chondrocytes grow and eventually lose division capacity.
TL;DR: Analysis of collagen type at each successive subculture until the time of cellular senescence has shown that a change in synthesis occurs from the cartilage-specific Type II collagen to a mixture of Type I collagen and the Type I trimer.
Journal ArticleDOI
The loss of phenotypic traits by differentiated cells. VI. Behavior of the progeny of a single chondrocyte.
TL;DR: If the progeny of a single, genetically programmed chondrocyte may or may not synthesize chondroitin sulfate, then extragenic sites in the cytoplasm or cell surface must influence the decision as to which cluster of "luxur" molecules the cell will synthesize.
Journal ArticleDOI
Simultaneous synthesis of types I and III collagen by fibroblasts in culture.
TL;DR: Specific antibodies against types I and III collagens and procollagens were used to localize these proteins in cultured human cells and indicate that the same cell makes both proteins.
Related Papers (5)
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
Paul D. Benya,Joy D. Shaffer +1 more