Journal ArticleDOI
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
Paul D. Benya,Joy D. Shaffer +1 more
TLDR
Using SDS-polyacrylamide gel electrophoresis of intact collagen chains and two-dimensional cyanogen bromide peptide mapping, this work demonstrated a complete return to the differentiated collagen phenotype and demonstrates a reversible system for the study of gene expression.About:
This article is published in Cell.The article was published on 1982-08-01. It has received 2296 citations till now. The article focuses on the topics: Collagen, type I, alpha 1 & Type I collagen.read more
Citations
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Journal ArticleDOI
Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation
TL;DR: Cultured autologous chondrocytes can be used to repair deep cartilage defects in the femorotibial articular surface of the knee joint.
Journal ArticleDOI
Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects.
TL;DR: The existence of many new and encouraging biological approaches to cartilage repair justifies the future investment of time and money in this research area, particularly given the extremely high socio-economic importance of such therapeutic strategies in the prevention and treatment of these common joint diseases and traumas.
Journal ArticleDOI
Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors
TL;DR: The characteristics of 3D cell culture systems in comparison to the two-dimensional monolayer culture are discussed, focusing on cell growth conditions, cell proliferation, population, and gene and protein expression profiles.
Journal ArticleDOI
Control of stem cell fate by physical interactions with the extracellular matrix.
Farshid Guilak,Daniel M. Cohen,Bradley T. Estes,Jeffrey M. Gimble,Wolfgang Liedtke,Christopher S. Chen +5 more
TL;DR: Some of the physical processes by which cues from the ECM can influence stem cell fate are reviewed, with particular relevance to the use of stem cells in tissue engineering and regenerative medicine.
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Multipotent mesenchymal stem cells from adult human synovial membrane
TL;DR: It is demonstrated that human multipotent MSCs can be isolated from the SM of knee joints and have the ability to proliferate extensively in culture, and they maintain their multilineage differentiation potential in vitro, establishing their progenitor cell nature.
References
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Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
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Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
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Role of cell shape in growth control
Judah Folkman,Anne Moscona +1 more
TL;DR: Cell shape was found to be tightly coupled to DNA synthesis and growth in nontransformed cells, suggesting a mechanism that is important in growth control of mammalian cells, and providing a more fundamental interpretation of such phenomena as density dependent inhibition of cell growth and anchorage dependence.
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The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.
John M. Kissane,Eli Robins +1 more
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Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture
TL;DR: It is found that there is no strict correlation between cell morphology and type of collagen synthesised in cartilage colonies kept in monolayer culture at low density.