Journal ArticleDOI
Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture
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TLDR
It is found that there is no strict correlation between cell morphology and type of collagen synthesised in cartilage colonies kept in monolayer culture at low density.Abstract:
WHEN chondrocytes from sternal or articular cartilage are kept in monolayer culture at low density, they eventually lose their cartilage phenotype1–4. Within four passages or approximately 1 month in culture they change from a polygonal or round to a flattened, amoeboid-like shape5–7, and instead of cartilage collagen (type II collagen8) they synthesise the genetically different type I collagen. It is not known whether there is a strict correlation between the occurrence of cell flattening and the change in collagen synthesis within individual cells. We have reported that preferentially flattened, fibroblast-like cells at the edge of cartilage colonies synthesise type I collagen, whereas round or polygonal chondrocytes generally synthesise type II collagen1–3. The change is nearly complete in a culture at a time when excessive flattening is observed4. Using an immunofluorescence double staining technique9,10, we have now found that there is no strict correlation between cell morphology and type of collagen synthesised.read more
Citations
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Journal ArticleDOI
Collagen type I antisense and collagen type IIA messenger RNA is expressed in adult murine articular cartilage
TL;DR: The absence of changes in collagen mRNA expression indicates that alteration of chondrocyte phenotype does not occur during the successful repair process after moderate proteoglycan depletion, which is not known what is the exact mechanism of matrix restoration.
Journal ArticleDOI
A specificity for cellular fibronectin in its effect on cultured chondroblasts
TL;DR: The results suggest that the effect of cellular fibronectin on the differentiated properties of chondroblasts relies on some unique feature not possessed by plasma fibronsectin, and thus is not solely dependent on its own ability to stimulate adhesion and spreading.
Journal ArticleDOI
Combined Jellyfish Collagen Type II, Human Stem Cells and Tgf-β3 as a Therapeutic Implant for Cartilage Repair
Marion Pugliano,Xavier Vanbellinghen,Pascale Schwinté,Nadia Benkirane-Jessel,Laetitia Keller +4 more
TL;DR: A new stem cell - based therapeutic active implant for cartilage repair that combines jellyfish collagen type II, human stem cells and TGF-β3 as a therapeutic implant to improve cartilage differentiation and repair is reported.
Journal ArticleDOI
Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus
Hyesun Lee,Hyesun Lee,Bogyu Choi,Hyo Jung Moon,Jiyeon Choi,Kwideok Park,Byeongmoon Jeong,Dong Keun Han +7 more
TL;DR: RD-modified F127 dimethacrylate (FM-RGD) hydrogel was investigated as a 3-dimensional culture matrix for chondrocytes and produced significant improvements in cell proliferation, DNA production, and viability while allowing the chondROcytes to maintain their original spherical phenotypes.
Journal ArticleDOI
Evaluation of the potential of novel PCL-PPDX biodegradable scaffolds as support materials for cartilage tissue engineering.
TL;DR: The present study demonstrates the potential, unknown until now, of PCL–PPDX blend scaffolds in the field of cartilage tissue engineering.
References
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Journal ArticleDOI
Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo.
H von der Mark,K von der Mark +1 more
TL;DR: Specific antibodies against skin and bone collagen and cartilage collagen are prepared for the study of differential collagen synthesis during development of the chick embryo by immunofluorescence.
Journal ArticleDOI
Changes in type of collagen synthesized as clones of chick chondrocytes grow and eventually lose division capacity.
TL;DR: Analysis of collagen type at each successive subculture until the time of cellular senescence has shown that a change in synthesis occurs from the cartilage-specific Type II collagen to a mixture of Type I collagen and the Type I trimer.
Journal ArticleDOI
The loss of phenotypic traits by differentiated cells. VI. Behavior of the progeny of a single chondrocyte.
TL;DR: If the progeny of a single, genetically programmed chondrocyte may or may not synthesize chondroitin sulfate, then extragenic sites in the cytoplasm or cell surface must influence the decision as to which cluster of "luxur" molecules the cell will synthesize.
Journal ArticleDOI
Simultaneous synthesis of types I and III collagen by fibroblasts in culture.
TL;DR: Specific antibodies against types I and III collagens and procollagens were used to localize these proteins in cultured human cells and indicate that the same cell makes both proteins.
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