Journal ArticleDOI
Relaxation kinetics of dimer formation by self complementary oligonucleotides
TLDR
Relaxation measurements on the kinetics of dimer formation by self complementary oligoribonucleotides of the form A(pA)n−1 (pU)n, 4 ≤ n ≤ 7.1 indicate longer helices grow more rapidly than they dissociate, with an activation energy roughly proportional to chain length.About:
This article is published in Journal of Molecular Biology.The article was published on 1971-12-14. It has received 389 citations till now. The article focuses on the topics: Reaction rate constant & Dimer.read more
Citations
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Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal ArticleDOI
Improved free-energy parameters for predictions of RNA duplex stability
Susan M. Freier,Ryszard Kierzek,John A. Jaeger,Naoki Sugimoto,Marvin H. Caruthers,Thomas Neilson,Douglas H. Turner +6 more
TL;DR: These parameters predict melting temperatures of most oligonucleotide duplexes within 5 degrees C, about as good as can be expected from the nearest-neighbor model.
Journal ArticleDOI
A General Purpose RNA-Cleaving DNA Enzyme
TL;DR: An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions, and its activity is dependent on the presence of Mg2+ ion.
Journal ArticleDOI
Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curves
TL;DR: The general equations and methods of analysis described in this paper should be of particular interest to laboratories that currently are investigating association and dissociation processes in nucleic acids that exhibit molecularities greater than two.
Journal ArticleDOI
A general model for genetic recombination.
TL;DR: A general model is proposed for genetic recombination, which proposes the hypothesis that recombination is initiated by a single-strand (or asymmetric) transfer, which may, after isomerization, become a two-stranded exchange.
References
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Journal ArticleDOI
Kinetics of renaturation of DNA.
James G. Wetmur,Norman Davidson +1 more
TL;DR: It is proposed that the mechanism of the reaction involves the joining of short, homologous sites on the two strands followed by a fast, reversible zippering reaction with forward rate constant kt, which explains the temperature and the GC dependence.
Journal ArticleDOI
A study of polyadenylic acid at neutral pH.
Marc Leng,Gary Felsenfeld +1 more
TL;DR: The structure of polyadenylic acid at neutral pH has been studied by an examination of the hypochromism of poly A and a series of oligoadenylates and it is shown that essentially complete thermal denaturation curves of almost all these materials can be obtained, and that they correspond to a non-co-operative denaturation process.
Journal ArticleDOI
Theory of ``Melting'' of the Helical Form in Double Chains of the DNA Type
TL;DR: In this article, the authors extended the treatment of the transition between the helical and random forms of the desoxyribose nucleic acid (DNA) molecule to include explicitly the dissociation into two separate chains and the consideration of the effects of the ends of the chains.
Journal ArticleDOI
Heats of the helix–coil transitions of the poly A–poly U complexes
TL;DR: The heats of the conformational transitions of poly (A + U) and poly(A + 2U) were determined in a twin‐cell differential thermal analysis microcalorimeter and found to be interpretable with fair success by a simple phenomenological argument which employs the concept of binding of counterions to polyelectrolytes.
Journal ArticleDOI
Helix formation by dAT oligomers. I. Hairpin and straight-chain helices.
TL;DR: Molecular weight measurements demonstrate that the two-chain helices are first converted into one-chain helixes at low temperatures, and then these melt at higher temperatures, which appears to be the conversion of hairpin to straight- chain helices by chain slippage.
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Co-operative non-enzymatic base recognition III. Kinetics of the helix—coil transition of the oligoribouridylic · oligoriboadenylic acid system and of oligoriboadenylic acid alone at acidic pH☆
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