Journal ArticleDOI
Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium.
Atsushi Arakaki,Mayu Goto,Mina Maruyama,Takuto Yoda,Masayoshi Tanaka,Ayana Yamagishi,Yasuo Yoshikuni,Tadashi Matsunaga,Tadashi Matsunaga +8 more
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TLDR
Magnetosome production is successfully enhanced by integrating a variation of magnetosome gene operons in the chromosome, which will allow the design of functional magnetosomes de novo and M. magneticum AMB‐1 may be used as a chassis for the designed magnetosom production.Abstract:
Integration of a large-sized DNA fragment into a chromosome is an important strategy for characterization of cellular functions in microorganisms. Magnetotactic bacteria synthesize intracellular organelles comprising membrane-bound single crystalline magnetite, also referred to as magnetosomes. Magnetosomes have gained interest in both scientific and engineering sectors as they can be utilized as a material for biomedical and nanotechnological applications. Although genetic engineering of magnetosome biosynthesis mechanism has been investigated, the current method requires cumbersome gene preparation processes. Here, the chromosomal integration of a plasmid containing ≈27 magnetosome genes (≈26 kbp region) in a non-magnetic mutant of Magnetospirillum magneticum AMB-1 using a broad-host-range plasmid is shown. The genome sequencing of gene-complemented strains reveals the chromosomal integration of the plasmid with magnetosome genes at a specific site, most likely by catalysis of an endogenous transposase. Magnetosome production is successfully enhanced by integrating a variation of magnetosome gene operons in the chromosome. This chromosomal integration mechanism will allow the design of functional magnetosomes de novo and M. magneticum AMB-1 may be used as a chassis for the designed magnetosome production.read more
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Journal ArticleDOI
Novel nanomaterial-organism hybrids with biomedical potential.
TL;DR: In this paper, a review summarizes recent advances in material-directed organism engineering and their outstanding biomedical applications, and three representative types of biomimetic mineralization are systematically introduced, including external mineralization, internal mineralization and genetic engineering mineralization.
Journal ArticleDOI
Why Does Not Nanotechnology Go Green? Bioprocess Simulation and Economics for Bacterial-Origin Magnetite Nanoparticles.
TL;DR: In this paper, the technical requirements and economic factors for setting up industrial production of magnetotactic bacteria (MTB)-derived nanomagnets were studied in the present paper.
Journal ArticleDOI
Nanomaterials for Biomedical Applications.
TL;DR: This special issue discusses nanostructured materials in different shapes and assembly scales suggested for healthcare applications, and the importance of using biomimetic 3D cellular aggregates as in vitro tumor models.
Book ChapterDOI
Molecular Imaging with Genetically Programmed Nanoparticles
TL;DR: This chapter discusses the advantages and challenges of gene-based contrast, using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection.
Book ChapterDOI
Molecular Imaging with Genetically Programmed Nanoparticles
TL;DR: In this article , the advantages and challenges of gene-based contrast are discussed using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection.
References
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Journal ArticleDOI
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Journal ArticleDOI
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TL;DR: This Review discusses the diverse properties of magnetosome biogenesis in other species of magnetotactic bacteria and considers the value of genetically 'magnetizing' non-magnetotacticacteria.