Restriction Maps of Five Autographa californica MNPV Variants, Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with Endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI.

TLDR: The restriction sites of Autographa californica nuclear polyhedrosis virus E2 DNA were mapped for the endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI to identify distinct AcMNPV variants, Trichoplusia ni, and Galleria mellonella genomes, which were distinct but with very similar genomes and protein structures.

Abstract: The restriction sites of Autographa californica nuclear polyhedrosis virus (Ac M NPV) E2 DNA were mapped for the endonucleases Sma I, Kpn I, Bam HI, Sac I, Xho I, and Eco RI. The restriction maps of four other Ac M NPV variants, Trichoplusia ni (Tn M NPV), and Galleria mellonella (Gm M NPV) genomes were determined and compared to the endonuclease cleavage maps of Ac M NPV E2 DNA. The viral structural polypeptides of Ac M NPV variants S3, E2, S1, M3, and R9 were the same when analyzed by polyacrylamide gel electrophoresis. The major structural polypeptides of Gm M NPV and Tn M NPV had the same pattern in polyacrylamide gels as did Ac M NPV structural polypeptides. Gm M NPV and Tn M NPV had several minor structural protein differences as compared with Ac M NPV. Ac M NPV variants, Tn M NPV, and Gm M NPV were distinct but with very similar genomes and protein structures. Images