Robust quantitative single-exposure laser speckle imaging with true flow speckle contrast in the temporal and spatial domains
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TLDR
The efficacy of the proposed true flow speckle contrast is demonstrated by imaging phantom flow at varying speeds, and the proposed LSCI analysis framework paves the way to estimate the true flow speed in the wide array of laser speckel contrast imaging applications.Abstract:
A systematic and robust laser speckle contrast imaging (LSCI) method and procedure is presented covering the LSCI system calibration, static scattering removal, and measurement noise estimation and correction to obtain a true flow speckle contrast Kf
2and the flow speed from single-exposure LSCI measurements. We advocate the use of K2 as the speckle contrast instead of the conventional contrast K, as the former relates simply to the flow velocity and is with additive noise alone. We demonstrate the efficacy of the proposed true flow speckle contrast by imaging phantom flow at varying speeds, showing that (1) the proposed recipe greatly enhances the linear sensitivity of the flow index (inverse decorrelation time) and the linearity covers the full span of flow speeds from 0 to 40 mm/s; and (2) the true flow speed can be recovered regardless of the overlying static scattering layers and the type of speckle statistics (temporal or spatial). The fundamental difference between the apparent temporal and spatial speckle contrasts is further revealed. The flow index recovered in the spatial domain is much more susceptible to static scattering and exhibit a shorter linearity range than that obtained in the temporal domain. The proposed LSCI analysis framework paves the way to estimate the true flow speed in the wide array of laser speckle contrast imaging applications.read more
Citations
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Journal ArticleDOI
Dilated Residual Learning With Skip Connections for Real-Time Denoising of Laser Speckle Imaging of Blood Flow in a Log-Transformed Domain
TL;DR: A dilated deep residual learning network with skip connections (DRSNet) is proposed, which shows good potential in real-time monitoring of blood flow for biomedical applications and image-quality evaluations of DRSNet with five raw speckle images outperform that of spatially average denoising with 20 raw specker images.
Journal ArticleDOI
Real-time video-rate perfusion imaging using multi-exposure laser speckle contrast imaging and machine learning
TL;DR: The presented MELSCI system allows for real-time acquisition and calculation of high-quality perfusion at 15.6 frames per second and shows higher signal dynamics compared to both single-exposure metrics, both spatially and temporally.
Journal ArticleDOI
Time varied illumination laser speckle contrast imaging.
TL;DR: In this paper, a varied illumination speckle contrast imaging method was proposed for high dynamic range flow rate imaging, which is advantageous in studying larger vessels and small arteries, and demonstrated the theory by simulations and ex vivo and in vivo measurements.
Journal ArticleDOI
Movement correction method for laser speckle contrast imaging of cerebral blood flow in cranial windows in rodents.
TL;DR: In this paper, the authors demonstrate a method to correct motion artifacts in LSCI signals measured in awake mice with cranial windows during sensory stimulation, showing that the corrected signal correlates better with independently measured blood volume and has a (59'±'6') higher signal-to-noise ratio.
Journal ArticleDOI
Depth resolution in multifocus laser speckle contrast imaging.
TL;DR: In this article, depth-resolved laser speckle contrast imaging (LSCI) is used to evaluate blood flow based on spatial or temporal statistics, but its accuracy is undermined by out-of-focus image blur.
References
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Book
Speckle Phenomena in Optics: Theory and Applications
Abstract: Comprehending as skillfully as conformity even more than further will allow each success. next to, the revelation as with ease as keenness of this speckle phenomena in optics theory and the applications can be taken as competently as picked to act. Better to search instead for a particular book title, author, or synopsis. The Advanced Search lets you narrow the results by language and file extension (e.g. PDF, EPUB, MOBI, DOC, etc).
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Model for laser Doppler measurements of blood flow in tissue.
Robert F. Bonner,Ralph Nossal +1 more
TL;DR: A theory is developed which relates quasi-elastic light scattering measurements to blood flow in tissue micro-vasculature and implies that the time decay of the photon autocorrelation function scales proportionally with cell size and inversely with mean translational speed.
Journal ArticleDOI
Laser speckle contrast imaging in biomedical optics
David A. Boas,Andrew K. Dunn +1 more
TL;DR: The underlying physics of speckle contrast imaging is reviewed, recent developments to improve the quantitative accuracy of blood flow measures are discussed and applications in neuroscience, dermatology and ophthalmology are reviewed.
Journal ArticleDOI
Laser speckle contrast imaging: theoretical and practical limitations
David Briers,Donald D. Duncan,E. R. Hirst,Sean J. Kirkpatrick,Marcus Larsson,Wiendelt Steenbergen,Tomas Strömberg,Oliver Thompson +7 more
TL;DR: LSCI is used primarily to map flow systems, especially blood flow, and its limitations and problems are investigated.
Journal ArticleDOI
Review of laser speckle contrast techniques for visualizing tissue perfusion.
Matthijs Draijer,Erwin Hondebrink,Ton G. van Leeuwen,Ton G. van Leeuwen,Wiendelt Steenbergen +4 more
TL;DR: The contribution of laser speckle contrast techniques to the field of perfusion visualization is presented and the development of the techniques are discussed.