Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
E Bier,Harald Vaessin,S Shepherd,K Lee,Kimberly McCall,S Barbel,Larry Ackerman,R Carretto,Tadashi Uemura,Ellsworth Grell +9 more
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TLDR
This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.Abstract:
A P-element vector has been constructed and used to generate lines of flies with single autosomal P-element insertions. The lines were analyzed in two ways: (1) the identification of cis-acting patterning information within the Drosophila genome, as revealed by a lacZ reporter gene within the P element, and (2) the isolation of lethal mutations. We examined 3768 independent lines for the expression of lacZ in embryos and looked among these lines for lethal mutations affecting embryonic neurogenesis. This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.read more
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Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TL;DR: The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.
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Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila
TL;DR: By targeted expression of the ey complementary DNA in various imaginal disc primordia of Drosophila, ectopic eye structures were induced on the wings, the legs, and on the antennae and support the proposition that ey is the master control gene for eye morphogenesis.
Journal ArticleDOI
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
G Friedrich,Philippe Soriano +1 more
TL;DR: Intercrossing of heterozygotes from 24 strains that express beta-galactosidase identified 9 strains in which homozygosity leads to an embryonic lethality, suggesting that a substantial proportion of mammalian genes identified by this approach are not essential for development.
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The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes.
Hugo J. Bellen,Robert W. Levis,Guochun Liao,Yuchun He,Joseph W. Carlson,Garson Tsang,Martha Evans-Holm,P. Robin Hiesinger,Karen L. Schulze,Gerald M. Rubin,Roger A. Hoskins,Allan C. Spradling +11 more
TL;DR: The Berkeley Drosophila Genome Project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.
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The Drosophila Tumor Suppressor Gene Warts Encodes a Homolog of Human Myotonic Dystrophy Kinase and Is Required for the Control of Cell Shape and Proliferation
TL;DR: The results raise the possibility that homozygous loss of the myotonic dystrophy kinase may contribute to the development of multiple pilomatrixomas and with other tumors including neurofibromas and parathyroid adenomas.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Genetic Transformation of Drosophila with Transposable Element Vectors
TL;DR: A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
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