Journal ArticleDOI
Segregation of the EBV-determined nuclear antigen (EBNA) in somatic cell hybrids derived from the fusion of a mouse fibroblast and a human Burkitt lymphoma line.
George Klein,Francis Wiener,Lore Zech,Harald Zur Hausen,Beverley M. Reedman,Beverley M. Reedman +5 more
TLDR
Four independently derived hybrids between the mouse fibroblast line A9 and the human, Burkitt‐lymphoma‐derived lymphoblastoid cell line Daudi were studied for the presence of the Epstein‐Barr virus (EBV) genome, theEBV‐determined nuclear antigen (EBNA), other EBV‐associated antigens, human surface immunoglobulin and the presenceof human chromosomes.Abstract:
Four independently derived hybrids between the mouse fibroblast line A9 and the human, Burkitt-lymphoma-derived lymphoblastoid cell line Daudi were studied for the presence of the Epstein-Barr virus (EBV) genome, the EBV-determined nuclear antigen (EBNA), other EBV-associated antigens, human surface immunoglobulin and the presence of human chromosomes. The four lines differed in the number of their EBV genomes. There was a parallelism between this number, as detected by c/RNA/DNA hybridization, and the frequency of EBNA-positive nuclei. None of the other EBV-antigens, EA, VCA or MA, was expressed at any time, either in the untreated hybrid cells or after IUDR-treatment. The hybrids did not carry detectable surface-associated immunoglobulin or EBV-receptors. The presence of the EBV genome was coincident with the maintenance of human chromosomes, but the hybrids that have lost detectable viral genomes and EBNA still contained a considerable number of human chromosomes, suggesting that the viral genome may be associated with a few chromosomes only.read more
Citations
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BookDOI
Transfer of cell constituents into eukaryotic cells
TL;DR: This paper presents results of microinjection of Somatic Cells with Micropipettes and PEG-Erythrocyte Ghost Mediated Microinjection into Tissue Culture Cells, which successfully transferred DNA into Plant Cells with the Ti-Plasmid as a Vector and Expression of Messenger RNAs into Xenopus Laevis Oocytes.
Book ChapterDOI
Latent Characteristics of Selected Herpesviruses
TL;DR: This chapter focuses on Herpes simplex virus, Epstein-Barr Virus, and the Lucke agent, which are well-studied representatives from each subgroup, and discusses the basic aspects of the latent state, particularly as they exist in the intact host.
Journal ArticleDOI
Human virus-infected target cells lacking HLA antigens resist specific T-lymphocyte cytolysis.
TL;DR: Daudi cells, originating from a human Burkitt's lymphoma line and lacking HLA products, are resistant to lysis by EBV-sensitised peripheral T lymphocytes from patients with infectious mononucleosis, indicating that HLA-lacking target cells should be resistant to immune T-ceil killing.
References
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Journal ArticleDOI
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
Beverley M. Reedman,George Klein +1 more
TL;DR: The ACIF test was used as a tool to trace the Epstein‐Barr virus genome at the cellular level to study the complementfixing antigens of human lymphoblastoid cell lines.
Journal ArticleDOI
Rosette-Forming Human Lymphoid Cell Lines. I. Establishment and Evidence for Origin of Thymus-Derived Lymphocytes
Journal ArticleDOI
Identification of human chromosomes by DNA-binding fluorescent agents
Torbjörn Caspersson,Torbjörn Caspersson,Lore Zech,Lore Zech,Clas B. Johansson,Clas B. Johansson,E.J. Modest,E.J. Modest +7 more
TL;DR: A convenient measuring technique has been developed for the rapid and accurate recording of fluorescence patterns in human metaphase chromosomes that should be particularly valuable for the identification of chromosomes 4–5 and the individual types in the 6–12 group.
Journal Article
Surface IgM-kappa Specificity on a Burkitt Lymphoma Cell In Vivo and in Derived Culture Lines
TL;DR: An exceptional Burkitt lymphoma patient, a 16-year-old boy, yielded tumor biopsy cells and derived tissue culture lines that displayed a strong surface accumulation of IgM heavy and kappa light chain specificities judged by direct membrane fluorescence and cytotoxicity tests.
Journal ArticleDOI
Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes.
Mikael Jondal,George Klein +1 more
TL;DR: Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma and it was concluded that B lymphocytes, in contrast to T lymphocyte, have receptors for EBV.
Related Papers (5)
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
Beverley M. Reedman,George Klein +1 more
Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes.
Mikael Jondal,George Klein +1 more