Journal ArticleDOI
Sensitization of T-Lymphocytes in Graves’ and Hashimoto's Diseases*
TLDR
To investigate the sensitization of T-lymphocytes to thyroid antigen in autoimmune thyroid disease, a modified migration inhibition factor test was employed using human peripheral T-LYmphocytes using patients with untreated Graves’ disease and Hashimoto's thyroiditis.Abstract:
To investigate the sensitization of T-lymphocytes to thyroid antigen in autoimmune thyroid disease, a modified migration inhibition factor test was employed using human peripheral T-lymphocytes. Mononuclear cells from the peripheral blood of patients with untreated Graves’ disease (GD) and Hashimoto's thyroiditis (HT) as well as from normal subjects were fractionated into E-rosetting (T-cell-enriched) and non-Erosetting (B cell- and monocyte-enriched) fractions. The migration index (MI) of T-lymphocytes from 20 patients with untreated GD and 13 patients with HT in response to either crude human thyroid antigen (88 /μg/0.5 ml) or solubilized TSH receptor antigen (20 μg/0.5 ml) was significantly inhibited compared to the unaffected migration of T-lymphocytes from 24 normal subjects. There was no correlation between these results and any of the thyroid autoantibodies. In GD patients taking propylthiouracil, the MI of T-lymphocytes in response to the thyroid antigens was inhibited in only 4 of 9 cases. The MI...read more
Citations
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Journal ArticleDOI
Immunology of autoimmune thyroid diseases.
TL;DR: The immune system is "unwilling" to mount an immune assault on its own tissues, according to Ehrlich's view, but the immune system can be tricked into thinking that it has no choice but to attack itself.
Journal ArticleDOI
Autoimmune thyroiditis : predisposition and pathogenesis
TL;DR: In this review, recent developments in autoimmune hypothyroidism are summarized, only touching on the important insights gained from experimental autoimmune thyroiditis (EAT) and other animal models, detailed elsewhere.
Journal ArticleDOI
Suppressor T-Lymphocyte Deficiency in Graves’ Disease and Hashimoto’s Thyroiditis*
TL;DR: There appears to be activity in normal T-lymphocytes which can suppress the ability of GD and HT T- lymphocytes to respond to the thyroid antigen, which is lacking in the GD andHT T-LYmphocytes themselves.
Journal ArticleDOI
Immunohistochemical characterization of intrathyroid lymphocytes in Graves' disease. Interstitial and intraepithelial populations.
TL;DR: Findings are consistent with the involvement of both helper-inducer and suppressor-cytotoxic T cells in a localized autoimmune reaction directed, at least in part, against the thyroid follicular epithelial cells.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal Article
An in Vitro Assay for Cellular Hypersensitivity in Man
TL;DR: A reproducible in vitro assay for delayed hypersensitivity in man is described and some clinical applications of this in vitro test are discussed.
Journal ArticleDOI
Studies on mediator production by highly purified human T and B lymphocytes.
TL;DR: In this article, the authors found that both T and B-cell populations made MIF; the production of MIF was antigen-specific using purified protein derivative of tuberculin, streptokinase-streptodornase and Candida antigens.